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A new experimental model to study shrimp allergy
Ivanéia Valeriano Nunes1, Camila Mattos Andrade2, Priscila Valera Guerra3
1Laboratório da Interação Parasita-Hospedeiro e Epidemiologia (LAIPHE), Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil; Department of Infectious Diseases, Medical Microbiology and Hygiene, University Hospital Heidelberg, Heidelberg, Germany.
Immunology Letters
|June 14, 2023
Summary
This study developed a new mouse model for shrimp allergy, crucial for testing new treatments. The model effectively mimics shrimp allergy symptoms, aiding research into food allergies.
Area of Science:
- Immunology
- Allergology
- Food Science
Background:
- Shrimp allergy is a significant health concern, often causing severe reactions like anaphylaxis.
- Existing research lacks systematic models to study shrimp allergy and explore novel therapeutic strategies.
Purpose of the Study:
- To establish a novel experimental model for shrimp allergy in mice.
- To facilitate the evaluation of new prophylactic and therapeutic interventions for shrimp allergy.
Main Methods:
- BALB/c mice were sensitized with Litopenaeus vannamei proteins and aluminum hydroxide, followed by a booster sensitization.
- An oral challenge was administered, and allergic responses were assessed through cytokine production (IL-4, IL-10), antibody levels (IgE, IgG1, IgA), and Passive Cutaneous Anaphylaxis assays.
- Immunoblotting and intestinal mucosal analysis were performed to characterize the allergic response.
Main Results:
- The experimental model successfully induced shrimp allergy, characterized by elevated IL-4 and IL-10 levels in lymph node cells.
- Significant detection of serum anti-shrimp IgE and IgG1, along with IgA in intestinal lavage, confirmed allergic sensitization.
- Passive Cutaneous Anaphylaxis and immunoblotting demonstrated an IgE-mediated response to multiple shrimp antigens, supported by intestinal changes.
Conclusions:
- The developed experimental protocol effectively models shrimp allergy in mice.
- This model serves as a valuable tool for assessing the efficacy of prophylactic and therapeutic strategies against shrimp allergy.
