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![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)
Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase
Published on: December 4, 2017
A photoactivated H2S donor based on a coumarin structure with real-time monitoring capability
Nianwei Wang1, Yuxi Fang1, Xuan Wang1
1Key Laboratory of Structure-Based Drug Design & Discovery, Ministry of Education, and School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, P. R. China. lidahong0203@163.com.
Abstract:
Attaining controlled, biocompatible H2S donors poses significant challenges. We developed Bhc-TCN-Ph, a photoactivated H2S donor derived from 6-bromo-7-hydroxycoumarinmethyl thiocarbonate. Upon 365 nm light exposure, COS is released, generating H2S and coumarin fluorescence for visualization. This process produces no electrophilic by-products. In vitro evaluations reveal good cytochemical and cytocompatibility characteristics.
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