SiC Electrochemical Sensor Validation for Alzheimer Aβ₄₂ Antigen Detection.

Area of Science:

• Biomedical Engineering
• Neuroscience
• Materials Science

Background:

• Alzheimer's disease (AD) diagnosis is often delayed until symptoms are severe, limiting treatment efficacy.
• Current diagnostic methods lack speed and reliability for early detection, impacting patient quality of life and caregiver burden.
• Developing rapid, effective sensors for early AD detection is crucial for potentially reversing disease progression.

Purpose of the Study:

• To validate the use of a Silicon Carbide (SiC) electrode for detecting amyloid-beta 42 (Aβ42), a biomarker for Alzheimer's disease.
• To establish a novel electrochemical sensor for early AD detection.
• To compare the performance of SiC electrodes against traditional gold (Au) electrodes for Aβ42 detection.

Main Methods:

• Fabrication and functionalization of both SiC and Au electrodes.
• Immobilization of Aβ1-28 antibodies onto both electrode types.
• Electrochemical analysis using Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS).
• Detection of a 0.5 µg·mL⁻¹ Aβ42 concentration in a 0.1 M buffer solution.

Main Results:

• A repeatable electrochemical peak correlated with the presence of Aβ42 was observed using the SiC electrode.
• The SiC-based sensor demonstrated potential for detecting the target biomarker.
• Validation experiments confirmed the SiC electrode's capability in sensing Aβ42.

Conclusions:

• A fast and reliable SiC-based electrochemical sensor for Alzheimer's disease detection has been successfully constructed.
• This novel SiC electrode approach shows promise for the early detection of Alzheimer's disease.
• Further development of this sensor could significantly improve early AD diagnosis and management strategies.