Multi working mode SPR chip laboratory for high refractive index detection.

Area of Science:

• Optoelectronics and Photonics
• Biochemical Sensing
• Microfluidics

Background:

• Fiber Surface Plasmon Resonance (SPR) chip laboratories are increasingly vital for biochemical detection.
• Existing SPR chips often have limitations in analyte detection range and channel capacity.
• There is a need for adaptable SPR platforms to meet diverse analytical requirements.

Purpose of the Study:

• To propose and demonstrate a multi-mode SPR chip laboratory based on microstructure fiber.
• To enhance the versatility of SPR chips for various detection scenarios.
• To develop a platform capable of high refractive index detection and multi-analyte sensing.

Main Methods:

• Integration of microfluidic devices (PDMS) with novel detection units (bias three-core fiber and dumbbell fiber).
• Utilizing different cores of bias three-core fiber to activate specific detection areas on the dumbbell fiber.
• Implementing distinct working modes: high refractive index detection, multi-channel detection, and temperature compensation.

Main Results:

• The chip successfully operates in high refractive index detection mode, covering a range of 1.571-1.595.
• In multi-channel mode, dual parameter detection of glucose and GHK-Cu was achieved with high sensitivities (4.16 nm/(mg/mL) and 9.729 nm/(mg/mL), respectively).
• The system demonstrated the capability to switch to a temperature compensation mode.

Conclusions:

• The proposed multi-mode SPR chip laboratory, based on microstructured fiber, offers a flexible and adaptable sensing platform.
• This technology provides a new pathway for developing portable testing equipment capable of detecting multiple analytes.
• The chip meets diverse detection requirements, enhancing the utility of SPR in biochemical analysis.