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Updated: Jul 24, 2025

Scanning Electron Microscopic Evaluation of Surface Defects of Remover Retreatment File After Single and Multiple Uses
Published on: October 11, 2024
An in vitro comparison of alterations in surface topographies of three different rotary files after root canal
Prem Sai Parepalli1, T B V G Raju1, P Krishna Prasad1
1Department of Conservative Dentistry and Endodontics, Sree Sai Dental College and Research Institute, Srikakulam, Andhra Pradesh, India.
Objectives:
The objective of the study was to evaluate and compare the surface topographies of WaveOne Gold (WOG), FlexiCON X1, and EdgeOne Fire (EOF) reciprocating files before and after root canal instrumentation using different irrigating solutions.
Materials And Methods:
Forty-eight extracted mandibular molars were selected and randomly assigned into three groups (n = 16) based on the file system used and each group was divided into two subgroups based on the irrigants used during root canal treatment. (Group-1: WOG; Group-2: FlexiCON X1; Group-3: EOF; Subgroup-A: 3% sodium hypochlorite [NaOCl] +17% ethylenediaminetetraacetic acid [EDTA]; Subgroup-B: Citra wash as irrigating solutions). The surface topography of the files was analyzed using atomic force microscope before and after instrumentation. Average roughness and root mean square roughness were calculated. Independent and paired t-tests and one-way analysis of variance with Tukey's post hoc were used for statistical analysis.
Results:
Atomic force microscopy results showed an increase in surface roughness after instrumentation, EOF showing the highest roughness. Citra wash showed more roughness compared to NaOCl and EDTA together. However, the surface roughness between the experimental groups WOG and EOF are statistically insignificant and this insignificance was also seen among subgroups (P>0.05).
Conclusion:
Instrumentation with various irrigating solutions affected the surface topography of EOF, WOG, and FlexiCON X1 reciprocating files.

