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Phosphoproteomic and proteomic profiling in post-infarction chronic heart failure
Jiayue Wang1, Xiuhua Zhu1, Shenrui Wang1
1Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Frontiers in Pharmacology
|July 5, 2023
Summary
Post-infarction chronic heart failure involves significant molecular changes. This study identifies key proteins and kinases, like Bclaf1 Ser658, offering potential therapeutic targets for heart failure.
Area of Science:
- Cardiovascular Biology
- Proteomics
- Molecular Mechanisms of Disease
Background:
- Post-infarction chronic heart failure presents high morbidity and mortality.
- Limited evidence-based therapies exist for this condition.
- Phosphoproteomic and proteomic analyses offer insights into molecular underpinnings and therapeutic avenues.
Purpose of the Study:
- To investigate molecular changes in post-infarction chronic heart failure using phosphoproteomics and proteomics.
- To identify potential therapeutic targets for heart failure.
Main Methods:
- Global quantitative phosphoproteomic and proteomic analysis of rat left ventricular tissues.
- Bioinformatic analysis, including Protein-Protein Interaction Network and kinase-substrate enrichment analysis (KSEA).
- Identification of differentially expressed proteins and phosphorylated proteins.
Main Results:
- Identified 33 differentially expressed phosphorylated proteins (DPPs) and 129 differentially expressed proteins.
- DPPs were enriched in nucleocytoplasmic transport and mRNA surveillance pathways.
- Bclaf1 Ser658 identified as a key protein; 13 kinases were enhanced in heart failure, including PRKAA1, PRKACA, and PAK1.
Conclusions:
- Phosphoproteomic and proteomic profiles reveal significant changes in post-infarction chronic heart failure.
- Bclaf1 Ser658 may play a critical role in heart failure-associated apoptosis.
- PRKAA1, PRKACA, and PAK1 represent potential therapeutic targets for post-infarction chronic heart failure.
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