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Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

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Updated: Jul 24, 2025

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System

Published on: May 1, 2019

Efficient Microfluidic Downstream Processes for Rapid Antibody Hit Confirmation.

Ramona Gaa¹, Hannah Melina Mayer¹, Daniela Noack¹

¹Protein Engineering and Antibody Technologies, Merck Healthcare KGaA, Darmstadt, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|July 5, 2023

Summary

This summary is machine-generated.

Microfluidics enables rapid antibody discovery by isolating single antibody-secreting cells. This study details droplet-based sorting and gene recovery methods to overcome downstream challenges in biotherapeutic development.

Keywords:

Antibody hit discovery Microfluidics PCR recovery Single-cell cultivation Small-scale expression

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Published on: August 16, 2019

Area of Science:

Biotechnology
Immunology
Microfluidics

Background:

Microfluidics is increasingly used to study immune responses and develop biotherapeutics.
Technologies like picoliter droplets and nanopens allow high-throughput screening of antibody-secreting cells.
Challenges in downstream processing can limit success rates in antibody discovery.

Purpose of the Study:

To detail microfluidic-based methods for antibody discovery.
To explain droplet-based sorting and single-cell analysis techniques.
To address downstream processing challenges in antibody development.

Main Methods:

Utilizing droplet-based microfluidics for high-diversity screening of antibody-secreting cells.
Implementing single-cell polymerase chain reaction (PCR) for antibody gene recovery.
Employing single-cell sub-cultivation for supernatant analysis and confirmation.

Main Results:

Demonstration of droplet-based sorting for efficient isolation of antibody-secreting cells.
Successful recovery and reproduction of antibody genes from single cells.
Validation of single-cell culture for confirming antibody function.

Conclusions:

Microfluidic droplet sorting coupled with single-cell analysis offers a powerful approach for antibody discovery.
Detailed methods can help mitigate downstream processing challenges, improving biotherapeutic development.
This technology accelerates the identification of functional antibodies for therapeutic applications.