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Related Experiment Video

Updated: Jul 24, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

Published on: February 6, 2020

Modular Protein-DNA Cocrystals as Precise, Programmable Assembly Scaffolds.

Abigail R Orun¹, Ethan T Shields², Sara Dmytriw^2,3

¹Department of Chemistry, Colorado State University, 1301 Center Ave., Fort Collins, Colorado 80523, United States.

|July 5, 2023

Summary

This summary is machine-generated.

Scientists engineered protein-DNA cocrystals as programmable nanomaterial scaffolds. These isoreticular cocrystals can entrap DNA-binding molecules for applications in drug delivery and structural biology.

Keywords:

DNA-binding protein X-ray crystallography cocrystal design scaffold

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Last Updated: Jul 24, 2025

Synthesis of Information-bearing Peptoids and their Sequence-directed Dynamic Covalent Self-assembly

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Design and Synthesis of a Reconfigurable DNA Accordion Rack

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Published on: August 15, 2018

Area of Science:

Materials Science
Structural Biology
Nanotechnology

Background:

High-precision nanomaterials are crucial for entrapping DNA-binding molecules.
Applications include controlled drug delivery and scaffold-assisted structural biology.

Purpose of the Study:

To engineer protein-DNA cocrystals as programmable scaffolds for DNA-binding molecules.
To explore the potential of isoreticular cocrystal design for creating novel scaffolding materials.

Main Methods:

Designed isoreticular cocrystals with DNA-binding protein and cognate DNA blocks.
Utilized crystal symmetry for topology-preserving expansion of DNA stacks.
Investigated modular assembly and tunable DNA-DNA junctions.
Employed interpenetrating I222 lattice structure, similar to metal-organic frameworks (MOFs).

Main Results:

Achieved dependable crystallization in the I222 space group despite DNA modifications.
Demonstrated modular assembly with interchangeable DNA sequences for guest-specified scaffolding.
Showcased tunable DNA-DNA junctions accommodating varied overhang lengths and phosphorylation.
Successfully entrapped three distinct guest molecules within the scaffold crystals as a proof of concept.

Conclusions:

Isoreticular cocrystal design provides a programmable route to scaffolds for DNA-binding molecules.
The design principles can be applied to existing cocrystals for developing advanced scaffolding materials.
This approach offers a versatile platform for applications in drug delivery and structural biology.