Related Experiment Video
Updated: Jul 24, 2025

Synthesis of pH Dependent Pyrazole, Imidazole, and Isoindolone Dipyrrinone Fluorophores using a Claisen-Schmidt Condensation Approach
Published on: June 10, 2021
A dual-function fluorescent probe for the detection of pH values and formaldehyde
Bing Deng1, Leyuan Ding1, Shaoxiang Yang1
1Beijing Key Laboratory of Flavor Chemistry | China Food Flavor and Nutrition Health Innovation Center, Beijing Technology and Business University, Beijing, China.
This study introduces a dual-function fluorescent probe for detecting both pH and formaldehyde (HCHO). The probe offers a rapid, smartphone-based method for quantifying formaldehyde, even in distilled liquor samples.
Area of Science:
- Analytical Chemistry
- Chemical Sensing
- Fluorescent Probes
Background:
- Developing selective and sensitive probes for environmental and food safety monitoring is crucial.
- Existing methods for formaldehyde detection can be complex or time-consuming.
- Simultaneous detection of multiple analytes with a single probe is highly desirable.
Purpose of the Study:
- To develop a novel dual-function fluorescent probe (Probe 1) for the independent detection of pH and formaldehyde.
- To establish a smartphone-based detection system for formaldehyde using Probe 1.
- To validate the probe's efficacy in real-world samples, such as distilled liquor.
Main Methods:
- Synthesis and characterization of Probe 1, a dual-function fluorescent probe.
- Investigation of Probe 1's response to varying pH levels, observing colorimetric changes.
- Evaluation of Probe 1's fluorescence intensity changes in response to different formaldehyde concentrations.
- Utilizing a smartphone with a color detector to quantify formaldehyde based on RGB values.
- Establishing a quantitative relationship between probe signal (B*R/G) and formaldehyde concentration.
Main Results:
- Probe 1 exhibited distinct color changes (grey blue to light blue) with increasing pH.
- Fluorescence intensity of Probe 1 increased linearly with formaldehyde concentration.
- A linear functional relationship was established between the B*R/G value and formaldehyde concentration.
- The probe demonstrated successful detection of formaldehyde in a distilled liquor sample.
Conclusions:
- Probe 1 serves as an effective tool for the separate and sensitive detection of pH and formaldehyde.
- The developed smartphone-based method provides a rapid and convenient approach for formaldehyde quantification.
- Probe 1 shows potential for practical applications in real-world sample analysis, including food and beverage safety.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Fluorescence and Phosphorescence: Instrumentation
pH
Photoluminescence: Applications

