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Multi-parameter surface plasmon resonance instrument for multiple nucleic acid quantitative detection.

Huixiang Wang1, Honggang Wang1, Yafeng Huang1

  • 1College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China.

Biomedical Microdevices
|July 7, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces a rapid, label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection. The novel system enables quick and accurate identification of biomarkers like miRNA-21 and miRNA-141, crucial for cancer diagnostics.

Keywords:
BiosensorMultiple nucleic acidsNucleic acid hybridizationSurface plasmon resonance (SPR)

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Biosensing Technology

Background:

  • Multiplex nucleic acid assays are vital for disease diagnosis, environmental monitoring, and food safety.
  • Traditional methods face limitations including complex operations, long detection times, unstable labeling, and signal interference.
  • There is a need for rapid, sensitive, and user-friendly multiplex nucleic acid detection platforms.

Purpose of the Study:

  • To develop a real-time, rapid, label-free surface plasmon resonance (SPR) instrument for multiplex nucleic acid detection.
  • To address the challenges of multiplex detection and quantitative analysis in complex biological samples.
  • To provide a simple and efficient point-of-care testing (POCT) platform for small molecules like DNA and miRNA.

Main Methods:

  • Development of a multiparametric optical system using total internal reflection, a linear light source, prism, photodetector, and mechanical transmission.
  • Implementation of an adaptive threshold consistency correction algorithm to manage channel variations and enable quantitative comparisons.
  • Utilizing surface plasmon resonance (SPR) for label-free and amplification-free detection of target nucleic acids.

Main Results:

  • The SPR instrument successfully achieved label-free and amplification-free detection of miRNA-21 and miRNA-141 in 30 minutes.
  • The system demonstrated good repeatability and specificity, with a limit of detection (LOD) of 50 nM for target oligonucleotides.
  • The smallest detectable absolute amount of sample was approximately 4 pmol, highlighting high sensitivity.

Conclusions:

  • The developed SPR instrument offers a simple, efficient, and rapid solution for multiplex nucleic acid detection.
  • This technology has the potential to serve as a point-of-care testing (POCT) platform for various small molecules, including cancer biomarkers.
  • The label-free and amplification-free approach overcomes key limitations of traditional nucleic acid assays.