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Related Experiment Video

Updated: Jul 22, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

Simultaneous detection and quantification of multiple pathogen targets in wastewater.

Gouthami Rao¹, Drew Capone², Kevin Zhu¹

¹Department of Environmental Sciences and Engineering, Gillings School of Public Health, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Medrxiv : the Preprint Server for Health Sciences

|July 10, 2023

Summary

Wastewater surveillance can now track many pathogens simultaneously. This novel method expands public health monitoring beyond single targets, enhancing infectious disease detection and control.

Keywords:

multiplex pathogen detection wastewater surveillance

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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

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Last Updated: Jul 22, 2026

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A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

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Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Quantification and Whole Genome Characterization of SARS-CoV-2 RNA in Wastewater and Air Samples

Published on: June 30, 2023

Area of Science:

Environmental science
Microbiology
Public health

Background:

Wastewater-based epidemiology is crucial for public health surveillance.
Current methods often focus on single or few pathogens, limiting comprehensive monitoring.
Expanding pathogen targets can significantly enhance wastewater surveillance utility.

Approach:

Developed a novel quantitative multi-pathogen surveillance method targeting 35 bacteria, viruses, protozoa, and helminths.
Utilized TaqMan Array Cards (TAC) for simultaneous analysis.
Applied the method to concentrated wastewater samples from four Atlanta treatment plants (February-October 2020).

Key Points:

Detected a wide range of expected (e.g., enterotoxigenic E. coli, Giardia) and unexpected pathogens (e.g., Strongyloides stercoralis).
Identified SARS-CoV-2 along with less common targets like Acanthamoeba, Entamoeba histolytica, astrovirus, norovirus, and sapovirus.
Found stable concentrations for common pathogens in sewersheds serving ~2 million people.

Conclusions:

Demonstrated broad utility for expanding enteric pathogen surveillance scope in wastewater.
Quantification of diverse fecal pathogens can inform public health strategies.
Potential application in various settings for infection control and disease monitoring.