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Updated: Jul 23, 2025

Multimodal Optical Imaging Platform for Studying Cellular Metabolism
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Super-Resolution SERS Spectral Bioimaging.

Zachary D Schultz1, Deben N Shoup1, Abigail E Smith1

  • 1Department of Chemistry and Biochemistry, The Ohio State University, 100 W. 18 Avenue, Columbus, OH, USA 43210-1173.

Proceedings of Spie--The International Society for Optical Engineering
|July 11, 2023
PubMed
Summary
This summary is machine-generated.

Super-resolution imaging using surface-enhanced Raman scattering (SERS) with plasmonic nanoparticles allows precise localization of molecules. This nanotechnology breakthrough enables simultaneous super-resolved SERS imaging and spectral acquisition for biological cell analysis.

Keywords:
RamanSERSbiologicalimagingmicroscopyplasmonicssuper-resolution

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Area of Science:

  • Nanotechnology
  • Spectroscopy
  • Microscopy

Background:

  • Nanotechnology advances allow trace molecule detection via surface-enhanced Raman scattering (SERS) from plasmonic nanoparticles.
  • Super-resolution imaging techniques are crucial for visualizing nanoscale biological structures and molecular interactions.

Purpose of the Study:

  • To develop and present a novel technology for super-resolution imaging of plasmonic nanoparticles.
  • To enable simultaneous acquisition of super-resolved SERS images and their corresponding spectra.
  • To explore the application of this technique for gaining new insights into biological cells.

Main Methods:

  • Utilizing fluctuations in the SERS signal generated at the surface of plasmonic nanoparticles.
  • Applying localization microscopy techniques to analyze SERS signal fluctuations.
  • Developing a method for simultaneous super-resolved SERS imaging and spectral acquisition.

Main Results:

  • Achieved nanometer spatial resolution for localizing emitting molecules.
  • Demonstrated the capability to acquire both super-resolved SERS images and spectra concurrently.
  • Established a foundation for applying advanced SERS microscopy to biological systems.

Conclusions:

  • The developed technology offers a powerful tool for high-resolution molecular imaging in biological research.
  • Simultaneous imaging and spectral analysis provide comprehensive molecular information at the nanoscale.
  • This approach holds significant potential for advancing our understanding of cellular processes.