Related Experiment Video
Updated: Jul 23, 2025
Efficient Genome Editing of Mice by CRISPR Electroporation of Zygotes
Published on: December 16, 2022
Protocol to expand and CRISPR-Cas9 genomic edit murine MAIT cells for subsequent in vivo studies
Anastasia du Halgouet1, Aurélie Darbois2, Aurélia Alphonse2
1INSERM U932, PSL University, Institut Curie, 75005 Paris, France; National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.
Generating knockout mice for target molecules in specific T cell populations, without subset-specific promoters, is time-consuming and costly. Here, we describe steps for enriching mucosal-associated invariant T cells from the thymus, expanding them in vitro and performing a CRISPR-Cas9 knockout. We then detail procedure for injecting the knockout cells into wounded Cd3ε-/- mice and characterizing them in the skin. For complete details on the use and execution of this protocol, please refer to du Halgouet et al. (2023).1.
Generating knockout mice for target molecules in specific T cell populations, without subset-specific promoters, is time-consuming and costly. Here, we describe steps for enriching mucosal-associated invariant T cells from the thymus, expanding them in vitro and performing a CRISPR-Cas9 knockout. We then detail procedure for injecting the knockout cells into wounded Cd3ε-/- mice and characterizing them in the skin. For complete details on the use and execution of this protocol, please refer to du Halgouet et al. (2023).1.
