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Updated: Jul 23, 2025

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Applied Biosystems' GlobalFiler™ PCR Amplification Kit.

Georgia Williams¹, Megan M Foley², Kelly L Knight³

¹Forensic Science Program, George Mason University, Fairfax, VA, USA. gwilli25@gmu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|July 13, 2023

Summary

This summary is machine-generated.

The GlobalFiler™ PCR Amplification Kit enables sensitive amplification of human DNA, generating millions of copies of 24 genetic markers. This tool is crucial for forensic DNA analysis, even with minimal DNA samples.

Keywords:

Amplification CODIS DNA polymerase Forensic Genetics GlobalFiler™Loci Multiplex PCR STR ThermoFisher

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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

Polymerase Chain Reaction (PCR) is essential for DNA amplification.
Short Tandem Repeat (STR) analysis is a cornerstone of forensic DNA profiling.
Sensitive DNA amplification kits are critical for analyzing degraded or low-quantity samples.

Purpose of the Study:

To describe the preparation and PCR amplification of human forensic DNA samples.
To highlight the utility of the GlobalFiler™ PCR Amplification Kit.
To detail the process of amplifying 24 specific DNA markers.

Main Methods:

Utilizing the GlobalFiler™ PCR Amplification Kit, a 6-dye STR multiplex assay.
Combining a synthetic primer mix with DNA samples.
Subjecting samples to 29 or 30 cycles of thermal cycling (denaturing, annealing, extension).

Main Results:

The GlobalFiler™ kit demonstrates high sensitivity in amplifying human DNA.
Millions of copies of 24 STR loci are generated per reaction.
Detection of partial or full genetic profiles from diverse sample types is possible.

Conclusions:

The GlobalFiler™ PCR Amplification Kit is a valuable tool for forensic DNA analysts.
It enables the analysis of extremely low DNA quantities.
Effective amplification of forensic DNA samples is achievable with this kit.