Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An exploratory view into allelic drop-out of sequenced autosomal STRs.

Journal of forensic sciences·2024

Same author

Applied Biosystems™ PrepFiler™ Forensic DNA Extraction Kit (Manual and Semi-automated via AutoMate Express™).

Methods in molecular biology (Clifton, N.J.)·2023

Same author

Applied Biosystems' GlobalFiler™ PCR Amplification Kit.

Methods in molecular biology (Clifton, N.J.)·2023

Same author

Likelihood Ratio Calculation Using LRmix Studio.

Methods in molecular biology (Clifton, N.J.)·2023

Same author

Next-Generation Sequencing: ForenSeq™ DNA Signature Prep Kit with the Illumina MiSeq FGx.

Methods in molecular biology (Clifton, N.J.)·2023

Same author

Evaluation of Maximum DNA Yield from a New Noninvasive Buccal Collection Device Following Various Extraction Protocols.

Genetic testing and molecular biomarkers·2021

Search research articles

Related Experiment Video

Updated: Jul 22, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Published on: December 27, 2010

Rapid DNA Profile Development with Applied Biosystems RapidHIT™ ID System.

Megan M Foley¹

¹Department of Forensic Sciences, The George Washington University, Washington, DC, USA. mmfoley@gwu.edu.

Methods in Molecular Biology (Clifton, N.J.)

|July 13, 2023

Summary

The RapidHIT™ ID System generates a CODIS-compatible DNA profile in 90 minutes. This automated system simplifies forensic DNA analysis for law enforcement and disaster victim identification.

Keywords:

FBI DISC GeneMarker™ HID GlobalFiler™ Express NGM SElect™ Express Rapid DNA Rapid STR Analysis RapidHIT™ ID RapidINTEL™RapidLINK™

More Related Videos

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

Related Experiment Videos

Last Updated: Jul 22, 2026

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3

Published on: December 27, 2010

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Published on: March 30, 2015

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

Published on: June 14, 2017

Area of Science:

Forensic Science
Molecular Biology
Genetics

Background:

Traditional forensic DNA analysis can be time-consuming.
The need for rapid DNA profiling in various field applications is increasing.
Current systems may require specialized laboratory personnel.

Purpose of the Study:

To describe the RapidHIT™ ID System for rapid forensic DNA profile generation.
To detail the workflow from sample to profile using the system.
To introduce the associated software for analysis and management.

Main Methods:

Utilizes a direct amplification workflow targeting CODIS or ESS core loci.
Employs preloaded cartridges and a fully automated workflow.
Features a user-friendly interface for single sample processing.

Main Results:

Generates a CODIS-compatible Short Tandem Repeat (STR) profile in 90 minutes.
The RapidLINK™ Software provides initial analysis and flags non-compliant profiles.
Enables real-time results management for multi-instrument/multi-location systems.

Conclusions:

The RapidHIT™ ID System offers a rapid, automated solution for forensic DNA profiling.
The system is suitable for field use by non-laboratory personnel, such as law enforcement.
Facilitates timely database hits for investigations and aids in mass disaster victim identification.