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Related Experiment Video

Updated: Jul 23, 2025

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Published on: March 10, 2023

Mouse Choroid Proteome Revisited: Focus on Aging.

Donita Garland¹, James Harnly², Radha Ayyagari³

¹Harnly LLC, Bethesda, MD, USA.

Advances in Experimental Medicine and Biology

|July 13, 2023

Summary

Aging significantly alters choroidal proteomes, impacting extracellular matrix, immune function, and lipid metabolism. These changes are crucial for understanding age-related macular degeneration and inherited macular dystrophies.

Keywords:

Aging Bruch’s membrane Choroid Complement Extracellular matrix Immune system Lipid Mouse Proteome

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A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

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Last Updated: Jul 23, 2025

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

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Published on: March 10, 2023

Detecting Abnormalities in Choroidal Vasculature in a Mouse Model of Age-related Macular Degeneration by Time-course Indocyanine Green Angiography

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Published on: February 19, 2014

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Area of Science:

Ophthalmology
Gerontology
Biochemistry

Background:

Age is a primary risk factor for age-related macular degeneration (AMD).
Aging influences the clinical presentation of inherited macular dystrophies.
Understanding age-related molecular changes in the choroid is critical for eye health research.

Purpose of the Study:

To investigate the impact of aging on the proteome of the C57Bl6/J mouse choroid.
To identify specific biochemical pathways affected by aging in the choroid.

Main Methods:

Proteomic analysis of C57Bl6/J mouse choroids at two distinct ages.
Principal component analysis (PCA) to identify proteins contributing to age-related variance.
Differential protein expression analysis to find significant age-dependent changes.

Main Results:

Proteins most affected by aging were identified.
Key affected processes include alterations in extracellular matrix (ECM) composition.
Changes in immune system function and lipid metabolism were also implicated.

Conclusions:

Aging induces significant proteomic alterations in the mouse choroid.
These age-related changes in ECM, immunity, and lipid metabolism may contribute to AMD and other macular diseases.
Further research into these pathways could reveal therapeutic targets for vision preservation.