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Bimolecular Fluorescence Complementation
Published on: April 15, 2011
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Bimolecular Fluorescence Complementation (BiFC) Assay to Visualize Protein-Protein Interactions in Living Cells
Prabu Gnanasekaran1, Hanu R Pappu2
1Department of Plant Pathology, Washington State University, Pullman, WA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|July 14, 2023
Abstract:
Bimolecular fluorescence complementation (BiFC) assay is a method to visualize the protein-protein interaction in living cells. This technique is based on ability of the non-fluorescent fragment of fluorescent protein to form fluorescent complex when they are fused to two interacting proteins. In this chapter, we describe the widely used split yellow fluorescent protein (YFP) system to visualize the protein-protein interaction in plant cells.

