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Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

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Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
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Sample Preparation for Analysis: Advanced Techniques01:08

Sample Preparation for Analysis: Advanced Techniques

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Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
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Preparation of Samples for Electron Microscopy01:20

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To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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Related Experiment Video

Updated: Jul 23, 2025

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
07:12

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Published on: April 24, 2020

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Automated large volume sample preparation for vEM.

Erin S Stempinski1, Lucas Pagano2, Jessica L Riesterer3

  • 1Multiscale Microscopy Core, Oregon Health & Science University, Portland, OR, United States.

Methods in Cell Biology
|July 14, 2023
PubMed
Summary
This summary is machine-generated.

Automated specimen processing significantly speeds up sample preparation for volume electron microscopy (vEM). This method enhances heavy metal staining reproducibility and reduces hazardous chemical exposure for improved vEM data quality.

Keywords:
Array tomographyAutomated sample processingElectron microscopyFIB-SEMImage qualitySBF-SEMVolume-EM

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Area of Science:

  • Life Sciences
  • Microscopy
  • Biotechnology

Background:

  • Volume electron microscopy (vEM) use is increasing due to technological advancements.
  • Conventional sample preparation for vEM is time-consuming and involves hazardous chemicals.
  • Optimizing sample quality and minimizing artifacts are crucial for vEM studies.

Purpose of the Study:

  • To describe an automated method for preparing samples for vEM.
  • To evaluate the efficiency and quality of automated sample preparation compared to conventional methods.
  • To ensure compatibility with various vEM techniques like FIB-SEM, SBF-SEM, and AT.

Main Methods:

  • Utilized the mPrep™ ASP-2000™ automated specimen processor for sample preparation.
  • Developed protocols for vEM techniques including focused ion beam scanning electron microscopy (FIB-SEM), serial block face scanning electron microscopy (SBF-SEM), and array tomography (AT).
  • Compared automated processing results with a widely used conventional bench protocol for image quality analysis.

Main Results:

  • Automated processing significantly reduced sample preparation time.
  • Improved reproducibility of heavy metal staining was observed.
  • No compromise in data quality from backscattered electron imaging during SEM was found.
  • Minimized operator exposure to hazardous chemicals.

Conclusions:

  • Automated specimen processing offers a faster, safer, and reproducible alternative for vEM sample preparation.
  • The mPrep™ ASP-2000™ is effective for preparing samples compatible with multiple vEM techniques.
  • This automated approach maintains high data quality while improving workflow efficiency.