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Estimation of plasma catecholamines by high-performance liquid chromatography with electrochemical detection in
Abstract:
Plasma catecholamine levels were determined by high-performance liquid chromatography with electrochemical detection following alumina extraction. Mean individual recoveries of 50.5, 49.9 and 48% were obtained for norepinephrine, epinephrine and dopamine, respectively, and the limits of detection for each catecholamine were 0.15, 0.34 and 0.6 pmol/ml. Total analysis time for each plasma sample was approximately 1 h. Catecholamine levels were measured in plasma from control subjects and the ranges obtained were: norepinephrine, 0.33-5.98 pmol/ml; epinephrine, 0-4.77 pmol/ml; dopamine, 0-0.8 pmol/ml. When patients with subarachnoid haemorrhage were investigated, the ranges were found to be: norepinephrine, 0.23-7.27 pmol/ml; epinephrine, 0-4.91 pmol/ml; dopamine, 0-0.23 pmol/ml.