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Methods for sampling wound fluid from venous leg ulcers for molecular analyses: A scoping review
Jodi Christine McDaniel1, Bohyun Kim1, Dina Rose McGowan2
1College of Nursing, The Ohio State University, Columbus, Ohio, USA.
International Wound Journal
|July 16, 2023
Summary
Standardizing wound fluid sample collection is crucial for identifying reliable biomarkers for venous leg ulcer treatment. Current methods are inconsistent, hindering biomarker discovery and personalized care.
Area of Science:
- Wound healing research
- Biomarker discovery
- Molecular diagnostics
Background:
- Venous leg ulcer (VLU) exudate contains potential biomarkers for guiding treatment.
- Current molecular analysis of wound fluid lacks standardized pre-analytical methods.
- This heterogeneity impedes biomarker identification and clinical application.
Purpose of the Study:
- To review and summarize current methods for collecting, processing, and storing wound fluid samples in VLU research.
- To identify inconsistencies and gaps in existing methodologies.
- To highlight the need for standardized protocols.
Main Methods:
- Scoping review of studies published between 2012 and 2022.
- Searched PubMed, CINAHL, EMBASE, and Scopus databases.
- Included 19 studies focusing on wound fluid sampling techniques for VLUs.
Main Results:
- Five primary categories of wound fluid sampling methods were identified.
- Commonly, fluid was extracted from absorbent materials and then centrifuged.
- Significant heterogeneity was observed in sampling, processing, and storage methods, with no definitive patterns.
Conclusions:
- Existing wound fluid sampling methods in VLU research are highly heterogeneous.
- There is a critical need for standardized pre-analytical methods.
- Standardization will facilitate accurate biomarker comparisons and accelerate the development of targeted VLU treatments.
