Functionalization of pine kernel protein by pH-shifting combined with ultrasound treatments: Further improvement with...

As a novel plant protein, developing various aspects of pine kernel protein (PKP) functionality is essential to meet the demand for protein-rich foods. To achieve this, the PKP was functionalized by a combination of pH-shifting and ultrasound techniques. The solubility, emulsification and droplet stability of the PKP in the pH range suitable to food (pH 3 to 7) were further investigated. The pH 12-shifting was an effective strategy to increase the solubility of PKP under extreme acidic and neutral conditions, characterized by a higher content of β-sheets and random coils, a greater exposure of free sulfhydryl and hydrophobic groups. Furthermore, appropriate ultrasonic power (250 W) further improved the solubility of PKPs by disrupting intermolecular hydrogen and hydrophobic bonds. As the ambient acidity increased, the emulsions exhibited higher viscoelasticity and stronger protein interactions. Especially at pH 3, the oil droplets stabilized by U₂₅₀-PKP-12 (PKP treated with 250 W ultrasound-assisted pH 12-shifting) were homogeneously dispersed and surrounded by dense protein, maintaining small particle size and large electrostatic repulsion, and there was no apparent creaming or phase separation in the emulsions after 10 days of storage. Thus, the functionality of PKP after pH-shifting combined with ultrasonic treatments is further enhanced by increasing the environmental acidity.