Related Experiment Video

Updated: Aug 17, 2026

Bone Conditioned Medium: Preparation and Bioassay
07:18

Bone Conditioned Medium: Preparation and Bioassay

Published on: July 8, 2015

11.1K

Plasma-activated medium triggers immunomodulation and autophagic activity for periodontal regeneration

Shengfang Wang1,2, Peiyu Wang3,4,5, Rik Thompson3,4

  • 1Centre for Biomedical Technologies Queensland University of Technology Brisbane Queensland Australia.

Bioengineering & Translational Medicine
|July 21, 2023
PubMed

Insights

Plasma-activated medium (PAM) reduces inflammation in periodontitis by modulating macrophages. PAM also promotes tissue regeneration, suggesting its potential as a novel therapeutic for periodontal disease.

Area of Science:

  • Biomedical Engineering
  • Immunology
  • Periodontology

Background:

  • Periodontitis is characterized by inflammation and increased inflammatory macrophage infiltration.
  • Plasma-activated medium (PAM) is recognized for its role in regulating innate immunity, particularly macrophage function.

Purpose of the Study:

  • To investigate the immunomodulatory effects of PAM on macrophages.
  • To explore the potential of PAM in managing periodontitis.

Main Methods:

  • PAM was generated using an argon jet and applied to cultured macrophages.
  • Macrophage markers, Akt signaling pathway, autophagy, and proregenerative effects on periodontal ligament cells (PDLCs) were analyzed.

Main Results:

  • PAM stimulation significantly reduced proinflammatory macrophage markers.
  • PAM activated autophagy via the Akt signaling pathway.
  • PAM-treated macrophages enhanced osteogenesis/cementogenesis markers and mineralization in PDLCs.

Conclusions:

  • PAM exhibits significant immunomodulatory and proregenerative effects.
  • PAM demonstrates potential as a therapeutic agent for periodontitis.
Keywords:
autophagic activityimmunoregulationperiodontal regenerationplasma‐activated medium (PAM)

More Related Videos

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils07:15

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Published on: January 21, 2020

11.5K
Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model06:14

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Published on: February 17, 2023

4.1K

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...