Light and electron microscopic immunocytochemistry of proenkephalin-derived peptides in septal neurons

Specific antibodies against different opioid peptides derived from proenkephalin were used in light and electron microscopic studies to locate septal enkephalin-containing cells. Immunoreactive neurons were demonstrated only after pretreatment of the animals with colchicine. They were found in all three subdivisions of the lateral septal nucleus and in the ventral limb of the nucleus of the diagonal band. The medial septal nucleus and the dorsal limb of the nucleus of the diagonal band were devoid of immunoreactive cells. Electron microscopy showed intracellular enkephalin-like immunoreactivity with all antisera used in this study. The reaction was found in the cytoplasm, sometimes associated with the rough endoplasmic reticulum. Numerous enkephalin-immunoreactive nerve terminals and fibres were detected in the lateral septal nucleus, but axon terminals making contacts with enkephalin-immunoreactive neurons did not contain enkephalin-like immunoreactivity. The results suggest that some septal neurons synthesise proenkephalin. These cells may be either local interneurons or output cells to areas which receive innervation from the septal complex.