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Classification of formalin-fixed bladder cancer cells with laser tweezer Raman spectroscopy
Nga Tsing Tang1,2, Richard Robinson3,4, Richard D Snook1,2
1Department of Chemical Engineering and Analytical Science, School of Engineering, University of Manchester, Manchester, M13 9PL, UK. peter.gardner@manchester.ac.uk.
The Analyst
|July 24, 2023
Summary
Early bladder cancer detection is challenging due to subtle symptoms. This study shows Raman spectroscopy can identify invasive bladder cancer cells in urine with over 87% accuracy, offering a promising non-invasive diagnostic tool.
Area of Science:
- Biomedical Optics
- Cancer Diagnostics
- Spectroscopy
Background:
- Bladder cancer diagnosis is often delayed due to non-specific early symptoms.
- Detecting bladder cancer early is crucial for improving patient outcomes.
- Urine samples contain bladder cells, presenting an opportunity for non-invasive detection.
Purpose of the Study:
- To investigate the potential of Raman spectroscopy for identifying bladder cancer cells in urine.
- To develop a non-invasive method for early bladder cancer detection.
Main Methods:
- Raman spectroscopy was employed to analyze biochemical information from individual bladder cells.
- Laser tweezers were used for the isolation of single cells from urine samples.
- Principal component-canonical variates analysis (PC-CVA) with k-fold validation was utilized for data analysis.
Main Results:
- Raman spectroscopy successfully characterized various bladder cell types found in urine.
- Invasive bladder cancer cells were identified with an accuracy exceeding 87%.
- The method demonstrated high specificity and sensitivity in distinguishing cancerous cells.
Conclusions:
- Raman spectroscopy combined with laser tweezers shows significant potential for non-invasive early bladder cancer detection.
- This technique can accurately identify invasive bladder cancer cells within urine samples.
- Further development could lead to a routine diagnostic test for bladder cancer.
