Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Transmission Electron Microscopy01:15

Transmission Electron Microscopy

5.6K
In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
5.6K
Atomic Force Microscopy01:08

Atomic Force Microscopy

3.4K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.4K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

4.3K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
4.3K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

9.3K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.3K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

10.5K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
10.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predictive drift compensation of multi-frame STEM via live scan modification.

Ultramicroscopy·2026
Same author

Relaxing Direct Ptychography Sampling Requirements via Parallax Imaging Insights.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same author

Beyond Contrast Transfer: Spectral SNR as a Finite-Dose Metric for STEM Phase Retrieval.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same author

Co-producing data-intensive research with an underserved group: a case study and evaluation identifying pathways to impact.

International journal of population data science·2026
Same author

Social Vulnerability Postincarceration: Analyzing Social Networks, Social Integration, and Loneliness Among Older Adults.

The Gerontologist·2025
Same author

Willingness to participate in placebo-controlled surgical trials of the knee.

The bone & joint journal·2024
Same journal

Gradient-Based Experimental Design for Defect Detection in MoS2 Including Emission Potentials for Thermal Diffuse Scattering.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

An Automated Atom Probe Tomography Cluster Detection Approach Using Transfer Learning.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Correlative Light and Electron Microscopy Visualization of Helicobacter pylori in Human Saliva.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Integrating Morpho-Anatomy and Histochemistry to Characterize Native Brazilian Eugenia Species.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Polyalthia Longifolia Induced Apoptosis via miR-484 Downregulation: A Multimodal In Situ Microscopy, In Vitro, and In Vivo Investigation.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same journal

Rhythmic Pattern of the Ovarian Development in Posthatching Japanese Quail (Coturnix coturnix japonica): Histological, Ultrastructural, and Immunohistochemical Study.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
See all related articles

Related Experiment Video

Updated: Jul 22, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.8K

Interlacing in Atomic Resolution Scanning Transmission Electron Microscopy.

Jonathan J P Peters1,2, Tiarnan Mullarkey1,2,3, James A Gott4,5

  • 1Advanced Microscopy Laboratory, Centre for Research on Adaptive Nanostructures and Nanodevices (CRANN), Trinity College Dublin, Dublin D02 DA31, Ireland.

Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
|July 25, 2023
PubMed
Summary
This summary is machine-generated.

This study introduces an interlaced imaging method for scanning transmission electron microscopy. This technique achieves faster frame rates with minimal image quality loss and superior strain precision, even with existing hardware.

Keywords:
high frame-rate STEMinterlacingscanning transmission electron microscopystrain precision

More Related Videos

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.3K
Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

7.4K

Related Experiment Videos

Last Updated: Jul 22, 2025

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

3.8K
Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
08:04

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

Published on: March 12, 2017

9.3K
Picometer-Precision Atomic Position Tracking through Electron Microscopy
15:04

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

7.4K

Area of Science:

  • Materials Science
  • Microscopy
  • Physics

Background:

  • Scanning transmission electron microscopy (STEM) requires fast frame rates for applications like dose control, in situ experiments, and reducing scan distortions.
  • Current methods for increasing frame rates often compromise image quality or necessitate expensive hardware upgrades.

Purpose of the Study:

  • To present an interlaced imaging approach for enhancing STEM frame rates.
  • To demonstrate minimal loss of image quality with faster acquisition.
  • To show improved strain precision compared to other methods.

Main Methods:

  • Implementation of an interlaced imaging strategy on existing scan controllers.
  • Evaluation of image quality at increased frame rates.
  • Comparison of strain precision under varying electron doses and imaging techniques.

Main Results:

  • The interlaced imaging approach successfully increases frame rates with minimal impact on image quality.
  • This method is compatible with many existing scanning electron microscope controllers.
  • The interlacing technique offers the highest strain precision for a given electron dose.

Conclusions:

  • Interlaced imaging is an effective and accessible method for achieving high-speed STEM.
  • This approach balances frame rate enhancement with image fidelity.
  • It represents a significant advancement for in situ studies and precise measurements in STEM.