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Electrospinning Fibrous Polymer Scaffolds for Tissue Engineering and Cell Culture
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Tendon-derived matrix crosslinking techniques for electrospun multi-layered scaffolds.

Thomas L Jenkins1, Paula A Sarmiento Huertas1, Kentaro Umemori1

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA.

Journal of Biomedical Materials Research. Part A
|July 25, 2023
PubMed
Summary

Crosslinking tendon-derived matrix (TDM) to poly(ε-caprolactone) (PCL) scaffolds using UV or EDC methods enhances cell attachment and tendon-like matrix synthesis. This strategy improves TDM retention on scaffolds for better tendon repair.

Keywords:
carbodiimidecollagenextracellular matrixhuman adipose stem cellsmesenchymal stem celltissue engineeringultraviolet radiation

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Tendon tears often result in incomplete healing and fibrosis, necessitating improved repair strategies.
  • Tissue engineering approaches utilize cell-seeded scaffolds with biomimetic factors to enhance tendon repair.
  • Effective retention of biomimetic factors on scaffolds is crucial for maximizing therapeutic benefits and minimizing risks.

Purpose of the Study:

  • To evaluate cross-linking methods for enhancing the retention of tendon-derived matrix (TDM) on electrospun poly(ε-caprolactone) (PCL) scaffolds.
  • To assess the impact of cross-linking on human adipose stem cell (hASC) behavior and tendon-like matrix synthesis.

Main Methods:

  • Tested ultraviolet (UV) and carbodiimide (EDC:NHS:COOH) cross-linking methods on PCL films with TDM.
  • Evaluated TDM retention, hASC spreading, and mechanical properties (modulus, stiffness) of cross-linked scaffolds.
  • Assessed hASC-induced tendon-like differentiation and analyzed scaffold composition using Fourier transform infrared spectroscopy.

Main Results:

  • No tested cross-linking method significantly retained more TDM than coating alone, but UV and EDC methods improved hASC spreading.
  • UV-crosslinked scaffolds exhibited increased modulus and stiffness compared to untreated or TDM-coated PCL scaffolds.
  • hASCs demonstrated enhanced spreading on UV-crosslinked scaffolds, and cross-linking did not alter characteristic tendon matrix peaks.

Conclusions:

  • Cross-linking TDM to electrospun PCL scaffolds improves tendon-like matrix synthesis.
  • UV or EDC cross-linking offers a viable strategy for enhancing TDM retention on PCL scaffolds.
  • This approach holds promise for improving outcomes in tendon tissue engineering and repair.