Related Experiment Video
Updated: Aug 9, 2026
10:57
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
Published on: October 13, 2019
[Behavior of the cell center during epithelial cell spreading]
Tsitologiia
|June 1, 1986
Summary
Centriole and cilium positioning in mouse renal epithelial cells changes with cell state, shifting from apical in situ to perinuclear in suspension and spread conditions. Microtubule organization also varies, suggesting cell state influences cytoskeletal dynamics.
Area of Science:
- Cell Biology
- Developmental Biology
- Microscopy
Context:
- Epithelial cells in mouse embryo renal tubules exhibit specific centriole localization.
- Centriole and cilium behavior is altered in suspended, single-adhering, and spread epithelial cells.
- Cellular state significantly impacts the spatial organization of centrioles and associated microtubules.
Purpose:
- To investigate the dynamic localization of centrioles and cilia in mouse renal epithelial cells under different culture conditions.
- To analyze the relationship between cell spreading, cell center, and microtubule organization.
- To understand the role of centrioles and microtubules in epithelial cell behavior and potential locomotion.
Summary:
- In situ, centrioles are apical and bear cilia extending into the lumen.
- Suspended cells show perinuclear centrioles without cilia, while spread cells have perinuclear centrioles with cilia extending apically.
- Microtubule organization around centrioles increases with cell spreading, but the cell center appears less involved in locomotion.
Impact:
- Provides insights into the plasticity of centriole and microtubule organization in epithelial cells.
- Highlights how cell-cell and cell-substrate interactions modulate cytoskeletal structures.
- Offers a basis for understanding cell polarity and migration mechanisms in renal development.
Related Concept Videos
01:09Cell Migration
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
01:19Cell Migration
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
01:13Actin Polymerization and Cell Motility
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate.
01:16Cell Motility through Blebbing
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
Blebbing Through the Matrix
In multicellular...
01:32Cytoskeletal Coordination in Cell Migration
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker proteins that...
01:13Centrioles and Centrosomes
Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or "prometaphase,"...
Near the end of the prophase, also called late prophase or "prometaphase,"...