Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Mitochondrial reticulum in limb skeletal muscle.

S P Kirkwood, E A Munn, G A Brooks

The American Journal of Physiology

|September 1, 1986

Summary

Mitochondria in rat skeletal muscles form a reticulum. This network structure was observed across slow-twitch oxidative and fast-twitch glycolytic fiber types, with higher mitochondrial volume density in soleus and deep vastus lateralis muscles.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Twelve weeks of endurance training increases FFA mobilization and reesterification in postmenopausal women.

Journal of applied physiology (Bethesda, Md. : 1985)·2010

Same author

Lactate shuttles in nature.

Biochemical Society transactions·2002

Same author

Lactate doesn't necessarily cause fatigue: why are we surprised?

The Journal of physiology·2001

Same author

Recovery of (13)CO2 during rest and exercise after [1-(13)C]acetate, [2-(13)C]acetate, and NaH(13)CO3 infusions.

American journal of physiology. Endocrinology and metabolism·2001

Same author

The effects of endurance training and exhaustive exercise on mitochondrial enzymes in tissues of the rat (Rattus norvegicus).

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2001

Same author

Autoregulation of glucose production in men with a glycerol load during rest and exercise.

American journal of physiology. Endocrinology and metabolism·2001

Area of Science:

Cell Biology
Muscle Physiology
Mitochondrial Research

Background:

Mitochondria are crucial for cellular energy production.
Skeletal muscle exhibits diverse fiber types with varying metabolic demands.
The structural organization of mitochondria within different muscle fiber types remains an area of investigation.

Purpose of the Study:

To investigate the three-dimensional morphology of mitochondria in different rat skeletal muscle fiber types.
To compare mitochondrial volume density and surface-to-volume ratios across fiber types.

Main Methods:

High-voltage electron microscopy (1,500 kV) was employed.
Three rat skeletal muscles representing distinct fiber types (soleus, deep vastus lateralis, superficial vastus lateralis) were analyzed.
Stereological analysis quantified mitochondrial volume densities and surface-to-volume ratios.

Main Results:

A mitochondrial reticulum (network) was identified in all examined skeletal muscle fiber types.
Mitochondrial volume density was significantly higher in soleus and deep vastus lateralis muscles compared to the superficial vastus lateralis.
No significant differences in mitochondrial surface-to-volume ratios were found between fiber types.

Conclusions:

Mitochondria in mammalian limb skeletal muscle exist as an interconnected network or reticulum.
The observed differences in mitochondrial volume density correlate with the metabolic characteristics of the respective muscle fiber types.

Related Experiment Videos