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Optimal Extraction and Deproteinization Method for Mannoprotein Purification from Kluyveromyces marxianus
Ashraf Hajhosseini1, Anousheh Sharifan2, Zohreh Eftekhari3
1Science and Research Branch, Islamic Azad University, Tehran, Iran.
Iranian Biomedical Journal
|August 1, 2023
Summary
Hydrochloric acid (HCl) effectively deproteinizes Kluyveromyces marxianus mannoproteins, yielding highly purified mannan oligosaccharides. This method is optimal for impurity removal and future scale-up procedures in industrial applications.
Area of Science:
- Biochemistry
- Industrial Biotechnology
Background:
- Mannoproteins, specifically mannose-glycosylated proteins, are crucial in biological systems.
- They possess diverse industrial applications, necessitating efficient extraction and purification methods.
- Yeast cell-wall mannoproteins are a significant source for these valuable compounds.
Purpose of the Study:
- To evaluate different deproteinization methods for extracting mannan oligosaccharides from Kluyveromyces marxianus mannoprotein.
- To assess the efficiency of Sevage, trichloroacetic acid, and hydrochloric acid (HCl) methods.
Main Methods:
- Deproteinization of K. marxianus mannoproteins using Sevage, trichloroacetic acid, and HCl.
- Quantification of total nitrogen, crude protein, fat, carbohydrate, and ash content.
- Assessment of mannan oligosaccharide loss and deproteinization percentage.
Main Results:
- The HCl method yielded highly purified mannan oligosaccharide (95.4%).
- This method achieved the highest deproteinization rate (97.33 ± 0.4%).
- Mannan oligosaccharide loss was observed at 25.1 ± 0.6% with the HCl method.
Conclusions:
- Hydrochloric acid (HCl) is the most effective deproteinization method for removing impurities from K. marxianus mannoprotein.
- This study provides preliminary data to guide the scale-up of mannan oligosaccharide production.
