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Multicolor-Encoded DNA Framework Enables Specific and Amplified In Situ Detection of the Mitochondrial Apoptotic
Fang Yang1, Shunmei Li1, Xin Bi1
1Key Laboratory of Luminescence Analysis and Molecular Sensing, Ministry of Education, School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
This study introduces a multicolor-encoded tetrahedral DNA framework (meTDF) for sensitive, simultaneous detection of manganese superoxide dismutase (MnSOD) mRNA and cytochrome c (Cyt c) to monitor mitochondrial apoptosis. The meTDF nanoprobes enable intracellular tracking of apoptotic pathways and drug screening.
Area of Science:
- Biotechnology
- Molecular Biology
- Cellular Imaging
Background:
- Elucidating cellular signaling pathways, particularly mitochondrial apoptosis, is crucial but challenging.
- Accurate monitoring of upstream and downstream molecules in apoptosis requires sensitive detection methods.
Purpose of the Study:
- To develop a novel nanoplatform for simultaneous and sensitive detection of mitochondrial apoptosis markers.
- To enable real-time monitoring of intracellular apoptosis signaling pathways.
Main Methods:
- Synthesis of a multicolor-encoded tetrahedral DNA framework (meTDF) functionalized with catalytic hairpin assembly (CHA) probes.
- Utilizing meTDF for in vitro and intracellular detection of manganese superoxide dismutase (MnSOD) mRNA and cytochrome c (Cyt c).
Main Results:
- Achieved sensitive and simultaneous detection of MnSOD mRNA (3.7 pM) and Cyt c (0.23 nM) in vitro.
- Demonstrated high stability and biocompatibility of meTDF for efficient intracellular delivery and monitoring.
- Successfully detected drug-regulated mitochondrial apoptosis pathways in cells.
Conclusions:
- The meTDF nanoprobes offer a robust platform for sensitive, simultaneous detection of key apoptosis molecules.
- This technology facilitates the study of apoptotic mechanisms and the screening of therapeutic drugs.
