Nanozymatic Biofuel Cell-Enabled Self-Powered Sensing System for a Sensitive Immunoassay
Xin Luo1, Zhen Luo2, Shentian Li1
1Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Key Laboratory of Fermentation Engineering (Ministry of Education), National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Hubei Research Center of Food Fermentation Engineering and Technology, Hubei University of Technology, Wuhan 430068, P. R. China.
Analytical Chemistry
|August 9, 2023
Summary
This study introduces a novel self-powered sensing system (SPSS) using nanozymes instead of natural enzymes for enhanced performance. The developed system demonstrates sensitive prostate-specific antigen (PSA) detection, offering a promising alternative for biosensing applications.
Area of Science:
- Electrochemistry
- Nanotechnology
- Biomedical Engineering
Background:
- Enzymatic biofuel cells (EFCs) integrated into self-powered sensing systems (SPSS) show promise but are limited by natural enzyme instability.
- Nanozymes offer a stable alternative to natural enzymes, presenting an opportunity for improved EFCs in SPSS.
- Developing high-performance nanozymatic biofuel cells for SPSS remains a significant challenge.
Purpose of the Study:
- To develop an advanced nanozymatic biofuel cell-enabled SPSS for sensitive prostate-specific antigen (PSA) detection.
- To utilize nanozymes as replacements for natural enzymes to overcome inherent limitations.
- To demonstrate the efficacy of the developed system for practical biosensing applications.
Main Methods:
- Fabrication of a nanozymatic biofuel cell using Ir single atoms on nitrogen-doped carbon as the cathode and gold (Au) nanozymes as the anode.
- Integration of the nanozymatic biofuel cell into a self-powered sensing system (SPSS).
- Electrochemical characterization and performance evaluation of the nanozymatic biofuel cell for PSA detection.
Main Results:
- The nanozymatic biofuel cell exhibited superior electrochemical activity and stability compared to platinum/carbon (Pt/C) counterparts.
- The developed SPSS demonstrated a higher power output and open-circuit potential, beneficial for self-powered applications.
- Sensitive detection of PSA was achieved with a wide linear range (0.2–500 ng mL⁻¹) and a low detection limit (62 pg mL⁻¹).
Conclusions:
- The developed nanozymatic biofuel cell-enabled SPSS offers a high-performance platform for sensitive PSA detection.
- Nanozymes provide a robust and efficient alternative to natural enzymes in biofuel cell applications for SPSS.
- This work provides new insights into expanding the application scope of nanozymes in biosensing and self-powered systems.
