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Imaging Biological Samples with Optical Microscopy

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Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Related Experiment Video

Updated: Jul 19, 2025

Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats

Using Optical Coherence Tomography and Optokinetic Response As Structural and Functional Visual System Readouts in Mice and Rats

Published on: January 10, 2019

Optical Coherence Tomography: Imaging Visual System Structures in Mice.

Xiangxiang Liu^1,2, Yuan Liu², Richard K Lee³

¹Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing, China.

Methods in Molecular Biology (Clifton, N.J.)

|August 9, 2023

Summary

This summary is machine-generated.

Optical coherence tomography (OCT) allows high-resolution imaging of the rodent eye. This non-invasive technology monitors retinal thickness in vivo, aiding disease research and treatment evaluation.

Keywords:

In vivo imaging Nerve fiber layer thickness Noninvasive ocular imaging Optical coherence tomography Retinal thickness

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In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

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Published on: July 24, 2020

Area of Science:

Ophthalmology
Neuroscience
Biomedical Imaging

Background:

Optical coherence tomography (OCT) provides micron-scale resolution of anatomical structures.
OCT is a valuable tool for ophthalmologic and neurologic research.
Imaging the small murine eye presents unique challenges for high-resolution techniques.

Purpose of the Study:

To describe the application of OCT for high-resolution retinal imaging in rodents.
To highlight OCT's utility in monitoring retinal layer thickness longitudinally in vivo.
To demonstrate OCT's role in assessing disease severity and treatment efficacy in murine models.

Main Methods:

Utilizing optical coherence tomography (OCT) for in vivo imaging of rodent retinas.
Performing longitudinal monitoring of retinal layer thickness.
Quantifying retinal thickness in healthy and diseased murine models.

Main Results:

OCT successfully provides high-resolution structural imaging of the murine retina.
Longitudinal OCT imaging enables monitoring of retinal layer thickness changes over time.
Retinal thickness quantification in rodents offers insights into disease progression and therapeutic effects.

Conclusions:

OCT is a powerful, non-invasive imaging technology for rodent retinal research.
OCT facilitates in vivo, longitudinal assessment of retinal structure and thickness.
This technique is crucial for understanding disease mechanisms and evaluating treatments in murine models.