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[Construction of a replicative expression vector based on the porcine circovirus 2 replicon]
Xiaoxue Cai1, Jun Li1, Zhangxun Li1
1College of Veterinary Medicine, Southwest University, Chongqing 402460, China.
Sheng Wu Gong Cheng Xue Bao = Chinese Journal of Biotechnology
|August 16, 2023
Summary
A novel DNA vaccine vector, pCMVori, was developed using porcine circovirus 2 (PCV2) for enhanced replication in eukaryotic cells. This replicative vector significantly increased antigen gene expression, improving DNA vaccine efficacy.
Area of Science:
- Molecular Biology
- Vaccinology
- Virology
Context:
- DNA vaccine efficacy is directly linked to antigen gene expression levels.
- Improving expression requires plasmid vectors capable of replication within eukaryotic cells.
Purpose:
- To construct and evaluate a novel replicative DNA vaccine vector, pCMVori, based on the porcine circovirus 2 (PCV2) replicon.
- To compare the replication efficiency and gene expression of pCMVori with a non-replicative vector (pcDNA3.1).
Summary:
- A new vector, pCMVori, was engineered by incorporating the PCV2 replicon into pcDNA3.1, enabling replication in eukaryotic cells.
- Recombinant pCMVori and pcDNA3.1 vectors carrying the EGFP gene were transfected into PK-15 cells.
- pCMVori demonstrated 136% replication efficiency and significantly enhanced EGFP expression (40% higher transcription, 39.14% higher fluorescence intensity) compared to pcDNA3.1.
Impact:
- The pCMVori vector shows potential for developing more effective DNA vaccines due to its enhanced gene expression capabilities.
- This study provides a foundation for future pCMVori-based DNA vaccine development and optimization.
