Related Experiment Video
Updated: Jul 19, 2025
Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids
Published on: August 11, 2017
Protocol for designing and bioprinting multi-layered constructs to reconstruct an endothelial-epithelial 3D model
Eduardo Henrique Backes1, Laura Nicoleti Zamproni2, Lina Maria Delgado-Garcia2
1Department of Biochemistry, Escola Paulista de Medicina, Universidade Federal de São Paulo, Sao Paulo, São Paulo 04044020, Brazil; Laboratory of Molecular Neurobiology, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo 04039002, Brazil; Department of Materials Engineering (DEMa), Universidade Federal de São Carlos, São Carlos 13565905, Brazil; Graduate Program in Materials Science and Engineering, Universidade Federal de São Carlos, São Carlos 13565905, Brazil.
This study details a protocol for creating multi-cell 3D bioprinted constructs using two cell lineages. These adaptable 3D bioprinted models are useful for drug screening and disease modeling.
Area of Science:
- Biotechnology
- Tissue Engineering
- Regenerative Medicine
Background:
- 3D bioprinting advances tissue engineering.
- Complex multi-cell constructs are needed for advanced applications.
Purpose of the Study:
- To present a protocol for designing and creating a 3D bioprinted model with two distinct cell lineage layers.
- To enable the recreation of multi-cell constructs for various research applications.
Main Methods:
- Protocol for designing a 3D model with dual cell layers (A549 and HUVEC).
- Detailed steps for construct slicing, hydrogel handling, and bioprinting setup.
- Adaptable protocol for diverse cell types including primary cells, cell lines, and induced pluripotent stem cells (IPCs).
Main Results:
- Successful creation of a protocol for 3D bioprinting multi-cell constructs.
- Demonstration of adaptability to various cell models and research needs.
- Establishment of a reproducible method for complex tissue engineering.
Conclusions:
- The presented protocol facilitates the development of sophisticated 3D bioprinted tissue models.
- These models hold significant potential for applications in drug screening and disease modeling.
- The methodology supports advancements in personalized medicine and regenerative therapies.
