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Updated: Jul 19, 2025

Genome-wide Analysis of Aminoacylation Charging Levels of tRNA Using Microarrays
Published on: June 18, 2010
Protocol to identify amino acids bound to tRNA by aminoacylation using mass spectrometry
Marc Finkler1, Joshua Brandt1, Timothée Boutfol1
1Universität des Saarlandes, Biologische Experimentalphysik, Zentrum f. Biophysik, Naturwissenschaftlich-Technische Fakultät, B2 1, Campus, 66123 Saarbrücken, Germany.
Abstract:
tRNA-bound amino acids often need to be identified, for instance, in cases where different amino acids compete for binding to the same tRNA. Here, we present a mass-spectrometry-based protocol to determine the amino acids bound to tRNA by aminoacylation. We detail how to perform the aminoacylation reaction, the preparation of the aminoacyl-tRNA for measurement, and the mass spectrometric analysis. We use arginine acylation as an example; however, this protocol can be applied to any other amino acid.
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