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Updated: Jul 19, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Help! I need a WNT antibody. Help! Not just any antibody
1The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
Abstract:
The emergence of antibody based surrogate WNT molecules has revolutionized research exploiting organoid cultures. In this issue of Cell Chemical Biology, Post et al.1 present a refined collection of WNT mimetics with unprecedented WNT/β-catenin pathway activating characteristics. These mimetics hold significant promise for future therapeutic advancements.
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Western Blotting
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

