Quality evaluation of Keteling capsules based on fingerprinting, multicomponent quantification, and quantitative...

Area of Science:

• Analytical Chemistry
• Traditional Chinese Medicine (TCM) Analysis
• Pharmacognosy

Background:

• Keteling capsule (KC), a TCM derived from Ficus microphylla and chlorpheniramine maleate, is widely used for cough and asthma.
• Current quality evaluation methods for KC are insufficient for rapid and comprehensive assessment.

Purpose of the Study:

• To develop and validate advanced analytical techniques for efficient quality control of Keteling capsules.
• To establish a comprehensive quality evaluation strategy for KC using complementary methods.

Main Methods:

• High-performance liquid chromatography (HPLC) fingerprinting with systematic quantification.
• Electrochemical fingerprint analysis utilizing Belousov-Zhabotinsky oscillation principles.
• Multi-component quantification via the Multi-markers assay by the monolinear method (MAML) and FT-IR quantitative models.

Main Results:

• HPLC fingerprinting successfully graded 22 KC samples into eight distinct categories.
• Electrochemical fingerprinting provided reliable parameters for active ingredient content analysis.
• MAML demonstrated accuracy, and FT-IR models accurately predicted chlorogenic acid, isovitexin, and chlorpheniramine maleate levels.

Conclusions:

• The integrated approach of HPLC and electrochemical fingerprinting offers a robust strategy for KC quality control.
• These methods provide a dependable and efficient means to enhance the quality analysis of TCM.
• The study offers new insights for advancing quality control methodologies in the field of traditional Chinese medicine.