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Programming therapeutic probiotics by self-tunable sense-and-respond genetic circuits
Yuxin Liu1, Zhengming Zhu1, Ling Jiang2
1College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China.
Trends in Microbiology
|August 24, 2023
Summary
Engineered probiotics with sense-and-respond genetic circuits offer precise disease detection and targeted therapy delivery. These smart probiotics enhance therapeutic precision, effectiveness, and intelligence for improved health outcomes.
Area of Science:
- Microbiology
- Synthetic Biology
- Genetic Engineering
Background:
- Probiotics offer a platform for targeted therapeutic delivery.
- Current probiotic therapies can lack precision and responsiveness to disease states.
- Engineered genetic circuits can imbue cells with sensing and response capabilities.
Purpose of the Study:
- To develop engineered probiotics capable of sensing intracellular disease signals.
- To create self-tunable sense-and-respond genetic circuits for enhanced therapeutic precision.
- To demonstrate advances in engineering probiotics for intelligent therapeutic activities.
Main Methods:
- Designing and constructing novel sense-and-respond genetic circuits.
- Programming probiotics with these genetic circuits.
- Evaluating the sensing and therapeutic effector delivery capabilities of engineered probiotics.
Main Results:
- Successful engineering of probiotics with functional sense-and-respond genetic circuits.
- Demonstration of probiotics' ability to detect specific intracellular disease signals.
- Validation of targeted therapeutic effector delivery mediated by engineered circuits.
Conclusions:
- Engineered probiotics with sense-and-respond circuits represent a significant advancement in precision medicine.
- Self-tunable genetic circuits enhance the intelligence and effectiveness of probiotic therapeutics.
- This technology holds promise for developing next-generation smart probiotics for various diseases.
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