Related Experiment Video

Updated: Jul 18, 2025

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System
05:06

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

645

Multidrug-Resistant and Extensively Drug-Resistant Acinetobacter baumannii Causing Nosocomial Meningitis in the

Nadezhda K Fursova1, Mikhail V Fursov2, Evgeny I Astashkin1

  • 1Department of Molecular Microbiology, State Research Center for Applied Microbiology and Biotechnology, Territory "Kvartal A", 142279 Obolensk, Russia.

Microorganisms
|August 26, 2023
PubMed

Insights

Acinetobacter baumannii meningitis is a serious threat, with multidrug-resistant strains causing high mortality. This study identified prevalent genetic clones and diverse antimicrobial resistance and virulence genes in Moscow neuro-ICU patients.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Acinetobacter baumannii is a significant cause of healthcare-associated meningitis.
  • This pathogen is associated with multidrug resistance and high mortality rates.
  • Understanding its genetic diversity and resistance mechanisms is crucial for effective treatment.

Purpose of the Study:

  • To characterize the genetic makeup, antimicrobial resistance, and virulence factors of Acinetobacter baumannii strains causing meningitis.
  • To identify prevalent sequence types (ST) and international clones.
  • To investigate the diversity of antimicrobial resistance and virulence genes.

Main Methods:

  • Isolation and identification of Acinetobacter baumannii strains from meningitis patients.
  • Antimicrobial susceptibility testing using the Vitek-2 instrument.
  • Whole-genome sequencing (Illumina) to determine sequence types (ST), capsular types (KL), and lipooligosaccharide outer core locus (OCL).
  • Identification of antimicrobial resistance and virulence genes.

Main Results:

  • Thirty-seven Acinetobacter baumannii strains were analyzed from Moscow neuro-ICU patients (2013-2020), with a 37.8% mortality rate.
  • The prevalent sequence type was ST2, belonging to the international clone IC2.
  • Twenty-nine strains were multidrug-resistant (MDR) and eight were extensively drug-resistant (XDR), with various resistance genes identified.
  • Multiple virulence genes across nine functional groups were detected, indicating significant pathogenic potential.

Conclusions:

  • Acinetobacter baumannii meningitis exhibits heterogeneity in genetic clones, antimicrobial resistance, and virulence gene profiles.
  • The international clone IC2, particularly ST2, is dominant among these meningitis-causing strains.
  • The findings underscore the need for continued surveillance and tailored therapeutic strategies against Acinetobacter baumannii meningitis.
Keywords:
Acinetobacter baumanniiICUMDRXDRbiofilmmeningitisvirulence genes

More Related Videos

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization07:41

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization

Published on: August 4, 2023

3.5K
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii10:24

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

Published on: April 10, 2020

13.4K

Related Concept Videos

Acute Pyelonephritis II: Diagnostic Studies and Management01:28

Acute Pyelonephritis II: Diagnostic Studies and Management

Introduction:For diagnosing acute pyelonephritis, a comprehensive patient history is collected to identify symptoms such as dysuria, frequent or urgent urination, flank pain, or costovertebral angle (CVA) tenderness that may suggest a kidney infection.Physical ExaminationDuring the physical examination, CVA tenderness is assessed. This involves gentle percussion over the costovertebral angle, where tenderness often indicates a kidney infection.Diagnostic TestsUrinalysis: Used to identify white...
17
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
40
Pneumonia IV: Management01:28

Pneumonia IV: Management

The treatment of pneumonia varies based on its severity and the causative pathogen. Here is a structured approach to managing pneumonia, integrating pharmaceutical and supportive care strategies.
Bacterial Pneumonia Treatment
For bacterial pneumonia, antibiotics serve as the cornerstone of therapy. Initial treatment often begins with empirical antibiotics, tailored to the anticipated causative organism and adjusted based on culture results. Key antibiotic choices include:
359
Drug Delivery: Parenteral Route01:29

Drug Delivery: Parenteral Route

The parenteral route is a critical method of drug administration. It delivers compounds directly into the systemic circulation and bypasses the gastrointestinal tract. This approach is particularly advantageous for drugs that exhibit poor absorption or instability when administered orally.
There are three primary parenteral routes: intravenous (IV), intramuscular (IM), and subcutaneous (SC). The IV route introduces the drug directly into the bloodstream, ensuring immediate action. The IM route...
612