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Microbiota Profiling on Veterinary Faculty Restroom Surfaces and Source Tracking.

Hiba Jabri1, Simone Krings2, Papa Abdoulaye Fall3

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|August 26, 2023
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Summary
This summary is machine-generated.

This study developed a microbial source tracking database for veterinary settings. DNA sequencing identified aquatic, human, and animal sources contributing to restroom microbial communities, aiding contamination source investigation.

Keywords:
16S rDNA amplicon sequencingbiotopesdatabasemicrobial source trackingmicroorganismsrestrooms

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Area of Science:

  • Microbiology
  • Veterinary Science
  • Environmental Health

Background:

  • Microbial contamination in veterinary facilities poses risks.
  • Accurate source tracking is crucial for infection control.
  • Existing microbial databases may lack specificity for veterinary environments.

Purpose of the Study:

  • To develop and validate a microbial source tracking database for veterinary settings.
  • To investigate microbial communities in veterinary facility restrooms.
  • To identify potential sources of bacterial contamination.

Main Methods:

  • 16S rDNA amplicon sequencing of restroom surfaces.
  • Development of a locally curated, metadata-enriched source tracking database.
  • Linking microbiota profiling with database analysis for source identification.

Main Results:

  • Dominance of Actinobacteria, Bacteroidetes, Proteobacteria, and Firmicutes phyla.
  • Significant differences in bacterial distribution between male and female users.
  • Identification of aquatic, human, and various animal sources (bovids, insects, equids) linked to specific bacterial genera.

Conclusions:

  • DNA sequence-based source tracking is effective for microbial investigations in veterinary settings.
  • The developed database aids in identifying known and unknown bacterial contamination sources.
  • Further research is needed to explore the tool's broader utility.