Integrated Cu-Au stereo microelectrode arrays and microfluidic channels for the electrochemical detection of glucose

Noble and transition metal nanomaterials are widely used in glucose sensing. However, the fabrication of these sensors still suffers from complex nanomaterial synthesis process and unstable nanomaterial loading on sensing surfaces. Herein, a Cu-Au bimetallic microelectrode array was prepared via local electrochemical deposition and electrochemical reduction without the need for templates and additional nanomaterial preparation processes. Based on the COMSOL computational fluid study, the obtained microelectrode arrays combined with microfluidic channels allow the continuous and rapid detection of glucose. Large number of active sites on the surface of 3D nano-arrays contributes to excellent sensing performance for glucose, with good linear detection ranges in 10 µM to 4 × 10² µM and 4 × 10² µM to 4 × 10⁵ µM, and a low detection limit of 284 nM. The feasibility of sensor in real sample was verified by detecting glucose in beverages with good recoveries ranging from 95.50% to 104.31%.