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Zbtb16 mediates a switch between Fgf signalling regimes in the developing hindbrain.

Sami A Leino1,2, Sean C J Constable1, Andrea Streit2

  • 1Neural Development Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

Development (Cambridge, England)
|August 29, 2023
PubMed
Summary

The Zbtb16 transcription factor coordinates fibroblast growth factor (Fgf) signaling transitions during zebrafish hindbrain development. Loss of Zbtb16 disrupts Fgf signaling timing, affecting neurogenesis patterns.

Keywords:
Fgf signallingHindbrainNeurogenesisZbtb16Zebrafish

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Area of Science:

  • Developmental biology
  • Molecular genetics
  • Neuroscience

Background:

  • Tissue patterning relies on precisely timed extracellular signals.
  • Fibroblast growth factor (Fgf) signaling plays distinct roles in early and late zebrafish hindbrain development.
  • The coordination mechanism for stage-specific Fgf signaling remains unclear.

Purpose of the Study:

  • To investigate the role of the Zbtb16 transcription factor in coordinating Fgf signaling transitions.
  • To understand how Zbtb16 regulates the switch between early and late Fgf signaling roles.
  • To elucidate the impact of Zbtb16 on hindbrain patterning and neurogenesis.

Main Methods:

  • Zebrafish model system.
  • Analysis of Zbtb16 expression patterns.
  • Gene expression analysis (fgf3, fgf8, fgf20).
  • Assessment of neurogenesis patterns in wild-type and Zbtb16 mutant embryos.

Main Results:

  • Zbtb16 expression is dynamically regulated in the developing hindbrain, initially anterior and later confined to neural progenitors.
  • In Zbtb16 mutants, fgf3 downregulation is impaired, leading to prolonged Fgf signaling.
  • Disrupted Fgf signaling timing in Zbtb16 mutants results in aberrant neurogenesis spatial patterning.

Conclusions:

  • Zbtb16 is essential for the orderly transition of Fgf signaling roles during hindbrain development.
  • Zbtb16 acts as a key regulator coordinating stage-specific Fgf signaling.
  • Proper regulation of Fgf signaling by Zbtb16 is critical for correct hindbrain patterning and neurogenesis.