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Mixed-Mode Chromatography and Its Role in Monoclonal Antibody Purification
1National Institute for Bioprocessing Research and Training (NIBRT), Blackrock, Dublin, Ireland. john.milne@nibrt.ie.
Methods in Molecular Biology (Clifton, N.J.)
|August 30, 2023
Summary
Mixed-mode chromatography utilizes multiple interactions for protein purification, offering benefits for biopharmaceutical manufacturing. This technique is particularly valuable for purifying immunoglobulin G (IgG) subtype monoclonal antibodies.
Area of Science:
- Biopharmaceutical Manufacturing
- Chromatographic Separations
- Protein Purification
Background:
- The biopharmaceutical industry requires stringent purification of products for safety, quality, and efficacy.
- Chromatography is a critical downstream processing operation in biopharmaceutical manufacturing.
- Mixed-mode chromatography, leveraging multimode interactions, has gained attention for its purification capabilities.
Purpose of the Study:
- To review the concept and benefits of mixed-mode chromatography in purification strategies.
- To examine the application of mixed-mode chromatography for purifying IgG subtype monoclonal antibodies.
Main Methods:
- Review of mixed-mode chromatography principles.
- Discussion of its application in biopharmaceutical purification.
Main Results:
- Mixed-mode chromatography offers versatile purification options through combined interaction modes.
- It provides effective strategies for purifying complex biomolecules like monoclonal antibodies.
Conclusions:
- Mixed-mode chromatography is a valuable technique for biopharmaceutical purification, especially for monoclonal antibodies.
- Its application aligns with process intensification and the production of personalized medicines.
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