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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: Jul 17, 2025

An Orthotopic Model of Murine Bladder Cancer

An Orthotopic Model of Murine Bladder Cancer

Published on: February 6, 2011

Orthotopic Mouse Models of Urinary Bladder Cancer.

Ji-In Noh¹, Eun-Jung Ahn², Myung-Giun Noh¹

¹Department of Pathology, Chonnam National University Research Institute of Medical Science, Chonnam National University Hwasun Hospital and Medical School, Hwasun, Republic of Korea.

In Vivo (Athens, Greece)

|August 31, 2023

Summary

This summary is machine-generated.

The open group method showed higher tumor formation rates (69%) compared to the catheter group (47%) for creating mouse bladder cancer models. This study highlights the importance of well-designed orthotopic animal models for research.

Keywords:

Urinary bladder neoplasms animal models immunotherapy non-muscle invasive bladder neoplasms therapeutics

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Last Updated: Jul 17, 2025

An Orthotopic Model of Murine Bladder Cancer

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Published on: February 6, 2011

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

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Published on: December 1, 2013

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Mouse Bladder Wall Injection

Published on: July 12, 2011

Area of Science:

Urology
Oncology
Immunology

Background:

Urinary bladder cancer often recurs and progresses, with poor treatment outcomes when muscles are invaded.
Developing effective syngeneic mouse models is crucial for immunological research in bladder cancer.

Purpose of the Study:

To compare the efficacy of two methods for creating orthotopic bladder tumor mouse models: transurethral catheterization and transperitoneal incision.
To evaluate tumor development and characteristics in syngeneic mouse models generated by different techniques.

Main Methods:

Utilized six-week-old female C3H/HeNCrl mice and the MBT-2 bladder tumor cell line.
Two methods were employed: transurethral catheterization with poly-L-lysine pretreatment (catheter group) and transperitoneal incision with direct tumor cell injection (open group).
Postoperative status was monitored weekly using magnetic resonance imaging (MRI).

Main Results:

The open group exhibited a higher tumor development rate (69%) compared to the catheter group (47%).
Intravesical tumor formation was more prevalent in the open group (27 mice) than the catheter group (1 mouse).
Urinary obstruction events occurred later in the lower cell count group within the open method.

Conclusions:

The transperitoneal incision and direct injection method (open group) demonstrated consistent effectiveness in constructing a stable orthotopic bladder tumor model.
Well-designed orthotopic animal models are essential for advancing bladder cancer research.
The open group method is a reliable approach for generating syngeneic mouse models for bladder cancer immunology studies.