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Updated: Jul 17, 2025
Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
Published on: July 16, 2018
A colorimetric sensor array based on peroxidase activity nanozyme for the highly efficient differential sensing of
Xiaoyu Yang1, Zhichun Bi1, Chenghui Yin1
1College of Food Science and Engineering, Jilin University, Changchun 130025, PR China.
Abstract:
Tieguanyin (TGY) is one of top ten famous teas in China, but in the process of brand building there is the phenomenon of falsehood, thus harming the interests of consumers. To solve theadulterate problem of TGY, a colorimetric sensor array (CSA) based onperoxidase activity of nanozyme was constructed. Nanozymes can catalyze 3,3',5,5'-tetramethylbenzidine (TMB) to 3,3',5,5'-tetramethyl -[1,1'-bis(cyclohexyl)]-2,2',5,5'-tetraene-4,4'-diimine (oxTMB), while the tea polyphenols (TPs) can inhibit this process, and the degree of inhibition varies significantly with the reaction time. We selected two nanozymesand three reaction time points to construct CSA. It can successfully distinguish TPsin TGY. The discriminative analysiscan achieve: (1)distinction between TGY and adulterated tea, (2)discrimination of TGY in various seasons and seasonal adulteration in different degrees. The method constructed in this work is promising for both the class and quality differentiation of TGY and other teas with TPs as the main activity.
