Related Experiment Video
Updated: Jul 17, 2025
10:44
Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
Published on: July 1, 2016
11.5K
Dual techniques for trace copper determination: DES/Dithizone based liquid phase microextraction-flame atomic
Yağmur Kilinç1, Buse Tuğba Zaman2, Sezgin Bakirdere3
1Zonguldak Bülent Ecevit University, Institute of Science, Department of Environmental Engineering, 67100 Zonguldak, Türkiye.
Food Chemistry
|September 2, 2023
Summary
A new method efficiently concentrates copper ions using dithizone complexation. This technique enables accurate copper determination via flame atomic absorption spectrometry (FAAS) and digital image colorimetry (DIC) with excellent recovery rates.
Area of Science:
- Analytical Chemistry
- Environmental Science
Background:
- Accurate copper ion determination is crucial for environmental monitoring and quality control.
- Existing methods for copper preconcentration can be complex and time-consuming.
Purpose of the Study:
- To develop a streamlined sample preparation method for copper ion preconcentration.
- To validate the method using two distinct analytical techniques: flame atomic absorption spectrometry (FAAS) and digital image-based colorimetry (DIC).
Main Methods:
- Copper ions were complexed with dithizone (Cu-DZ).
- The Cu-DZ complex was extracted from aqueous samples in a single step.
- Analysis was performed using FAAS and a digital image-based colorimetry system measuring RGB color changes.
Main Results:
- The FAAS system achieved a low detection limit of 2.3 ng/mL over a broad linear range.
- The DIC system demonstrated a broad linear working range with a detection limit of 14.7 ng/mL.
- Spike recovery experiments in eucalyptus tea extracts showed high recovery rates (91-107%).
Conclusions:
- The developed single-step complexation and extraction method is effective for copper ion preconcentration.
- Both FAAS and DIC systems provide reliable and sensitive quantification of copper.
- This method offers a practical approach for copper analysis in complex matrices like herbal teas.
Related Concept Videos
01:18Effects of EDTA on End-Point Detection Methods
302
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
302
01:19Precipitation Titration: Endpoint Detection Methods
1.8K
In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
In the Volhard method, a standard excess of AgNO3 is first added to the...
In the Volhard method, a standard excess of AgNO3 is first added to the...
1.8K
01:12Voltammetric Techniques: Linear-Scan (E vs Time)
435
Polarography is a classical voltammetric technique used to analyze electrochemical reactions. This method applies a linear potential sweep to a dropping mercury electrode (DME), and the resulting current is measured. A dropping mercury electrode is commonly used as the working electrode in polarography. It consists of a capillary tube filled with mercury, where the tiny droplet forms at the tip. This droplet continuously drops from the capillary, creating a new electrode surface for each...
435
01:19Gas Chromatography: Types of Detectors-II
416
In gas chromatography, different detectors are employed to meet specific analytical needs. These detectors are often categorized based on their detection mechanisms and the types of compounds they are best suited to analyze. Thermal Conductivity Detectors (TCD), Flame Ionization Detectors (FID), and Electron Capture Detectors (ECD) represent common categories, each with unique operating principles and applications. However, beyond these, several other detectors are designed for more specialized...
416
00:56Extraction: Advanced Methods
482
Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
482
01:02Flame Photometry: Overview
659
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
659