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Author Spotlight: Advancing EVtrap for High-Throughput Proteomics in Disease Biomarker Discovery
Published on: October 27, 2023
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A Robust and Clinically Applicable Sample Preparation Protocol for Urinary Extracellular Vesicle Isolation Suitable
Leyla A Erozenci1,2, Irene V Bijnsdorp3, Sander R Piersma1
1Department of Medical Oncology, OncoProteomics Laboratory, Cancer Center Amsterdam, Amsterdam UMC, Location VUMC, Amsterdam, The Netherlands.
Methods in Molecular Biology (Clifton, N.J.)
|September 4, 2023
Summary
We developed a fast urinary extracellular vesicle (uEV) isolation method for proteomics. This technique enables noninvasive protein biomarker discovery, such as in prostate cancer detection.
Area of Science:
- Biochemistry
- Proteomics
- Urology
Background:
- Urinary extracellular vesicles (uEVs) contain valuable noninvasive protein biomarkers.
- Current uEV isolation methods are often complex and not suitable for clinical proteomics.
Purpose of the Study:
- To describe a rapid, clinically applicable uEV isolation protocol.
- To demonstrate its utility for proteomic characterization and biomarker discovery.
Main Methods:
- Detailed protocol for uEV isolation from urine.
- Proteomic analysis using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
- Differential proteome analysis comparing prostate cancer patients and controls.
Main Results:
- A user-friendly protocol for uEV isolation was established.
- Comprehensive proteome profiling of isolated uEVs was achieved.
- Distinct proteomic signatures were identified between prostate cancer patients and controls.
Conclusions:
- The described uEV isolation protocol is efficient and suitable for clinical proteomics.
- This method facilitates noninvasive biomarker discovery for diseases like prostate cancer.
- uEV proteomics offers a promising avenue for early disease detection and diagnosis.
