Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

52.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

48
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
48
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

65
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
65
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PRICE: direct and robust detection of microRNAs at single-nucleotide resolution.

Nature communications·2026
Same author

Ultrasensitive detection of clinical pathogens through a target-amplification-free collateral-cleavage-enhancing CRISPR-CasΦ tool.

Nature communications·2025
Same author

Fast response cathodic electrochemiluminescence sensor based on closed bipolar electrode for point-of-care blood glucose testing.

Talanta·2025
Same author

A signal-off Cas14a1-based platform for highly specific detection of methicillin-resistant Staphylococcus aureus.

Analytica chimica acta·2023
Same author

Fluorescent Probe Combined with Photoelectric Analysis Technology for Detection of <i>Escherichia coli</i>.

Biosensors·2023
Same author

Aptamer-based Cas14a1 biosensor for amplification-free live pathogenic detection.

Biosensors & bioelectronics·2022
Same journal

RETRACTED: Kim et al. The Angiogenesis Inhibitor ALS-L1023 from Lemon-Balm Leaves Attenuates High-Fat Diet-Induced Nonalcoholic Fatty Liver Disease Through Regulating the Visceral Adipose-Tissue Function. <i>Int. J. Mol. Sci.</i> 2017, <i>18</i>, 846.

International journal of molecular sciences·2026
Same journal

Correction: Mahmud et al. Thymoquinone Attenuates NF-κβ Signalling Activation in Retinal Pigment Epithelium Cells Under AMD-Mimicking Conditions. <i>Int. J. Mol. Sci.</i> 2025, <i>26</i>, 11473.

International journal of molecular sciences·2026
Same journal

Correction: Borovikov et al. The Twisting and Untwisting of Actin and Tropomyosin Filaments Are Involved in the Molecular Mechanisms of Muscle Contraction, and Their Disruption Can Result in Muscle Disorders. <i>Int. J. Mol. Sci</i>. 2025, <i>26</i>, 6705.

International journal of molecular sciences·2026
Same journal

Correction: Molagoda et al. Flavonoid Glycosides from <i>Ziziphus jujuba</i> var. <i>inermis</i> (Bunge) Rehder Seeds Inhibit α-Melanocyte-Stimulating Hormone-Mediated Melanogenesis. <i>Int. J. Mol. Sci.</i> 2021, <i>22</i>, 7701.

International journal of molecular sciences·2026
Same journal

Correction: Guo et al. Integrated Transcriptomic and Metabolomic Analysis Reveals the Molecular Regulatory Mechanism of Flavonoid Biosynthesis in Maize Roots Under Lead Stress. <i>Int. J. Mol. Sci.</i> 2024, <i>25</i>, 6050.

International journal of molecular sciences·2026
Same journal

Correction: Chang et al. Improvement of Carbon Tetrachloride-Induced Acute Hepatic Failure by Transplantation of Induced Pluripotent Stem Cells Without Reprogramming Factor c-Myc. <i>Int. J. Mol. Sci.</i> 2012, <i>13</i>, 3598-3617.

International journal of molecular sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 16, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K

CRISPR/Cas-Based Techniques for Live-Cell Imaging and Bioanalysis.

Shuo Huang1, Rui Dai2, Zhiqi Zhang1

  • 1College of Life Sciences, Hainan University, Haikou 570228, China.

International Journal of Molecular Sciences
|September 9, 2023
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas systems offer precise gene editing and are now advanced tools for live-cell imaging and bioanalysis. This review explores their mechanisms, applications, and future potential in biological and clinical research.

Keywords:
CRISPR/Casbioanalysisbioimagingnucleic acid analysisprotein analysis

More Related Videos

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

268
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

9.1K

Related Experiment Videos

Last Updated: Jul 16, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.2K
Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes
08:32

Genome-Wide CRISPR Screen for Unveiling Radiosensitive and Radioresistant Genes

Published on: May 23, 2025

268
Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation
08:47

Lighting Up the Pathways to Caspase Activation Using Bimolecular Fluorescence Complementation

Published on: March 5, 2018

9.1K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Bioimaging

Background:

  • CRISPR/Cas systems are renowned for gene editing accuracy, programmability, and cost-effectiveness.
  • Cas enzyme cleavage properties extend CRISPR/Cas applications beyond gene editing.
  • Emerging uses include live-cell imaging and advanced bioanalysis.

Purpose of the Study:

  • To review CRISPR/Cas systems' fundamental mechanisms and concepts.
  • To describe recent advances and design principles for CRISPR/Cas in live-cell imaging and bioanalysis.
  • To highlight applications in imaging and biosensing diverse molecular targets.

Main Methods:

  • Review of CRISPR/Cas system mechanisms.
  • Analysis of recent CRISPR/Cas-mediated techniques for live-cell imaging and bioanalysis.
  • Compilation of applications in molecular target imaging and biosensing.

Main Results:

  • CRISPR/Cas systems are versatile tools for gene editing, live-cell imaging, and bioanalysis.
  • Numerous applications exist for imaging and biosensing various biological and clinical targets.
  • Recent advances focus on design principles for enhanced performance.

Conclusions:

  • CRISPR/Cas systems are expanding into live-cell imaging and bioanalysis.
  • Further development can improve tool design for imaging and quantifying biological elements.
  • This review guides the optimal use of CRISPR/Cas in these fields.