Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Desensitization and Tachyphylaxis01:20

Desensitization and Tachyphylaxis

1.8K
Tachyphylaxis is described as a rapid decrease in response to a drug after repeated or continuous administration of the same drug dose. It is a phenomenon where the body becomes less responsive to a particular substance or intervention over time, requiring higher doses or stronger interventions to achieve the same effect. It results from adaptive changes in the body's receptors, signaling pathways, or physiological processes that occur in response to prolonged exposure to a stimulus.
1.8K
Psychological Responses to Stress01:20

Psychological Responses to Stress

74
Psychological responses to stress encompass the various cognitive and emotional reactions individuals experience when faced with challenging or threatening situations, such as a job loss. Prolonged exposure to stressors can disturb emotional balance, increasing negative emotions (e.g., anxiety and sadness) and diminishing positive emotions (e.g., joy and satisfaction). These persistent emotional shifts are associated with an increased risk of both physical illness and mental health issues, such...
74
Coping Strategies: Problem Focused01:27

Coping Strategies: Problem Focused

95
Coping strategies are methods people use to manage, tolerate, or reduce the effects of stressors. These strategies involve both behavioral and psychological actions to handle stressful situations. One common approach is problem-focused coping, which aims to change or eliminate the source of stress rather than merely addressing its consequences. This method involves taking direct action to resolve the issue causing stress.
For example, consider a student who struggles to understand their...
95
Stringent Response in E. coli01:23

Stringent Response in E. coli

31
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
31
Stress Response System01:21

Stress Response System

110
The stress response system, also known as the fight-or-flight response, is the body's automatic physiological reaction to perceived threats. Hans Selye introduced the concept of General Adaptation Syndrome (GAS) to describe the predictable pattern of changes that occur in response to stress. GAS consists of three sequential stages: alarm, resistance, and exhaustion. This model helps explain how chronic stress can contribute to health problems.
Alarm stage
In the alarm stage, the body's...
110
Robbers Cave04:49

Robbers Cave

14.3K
During the 1950s, the landmark Robbers Cave experiment demonstrated that when groups must compete with one another, intergroup conflict, hostility, and even violence may result. At the Oklahoman summer camp, two troops of boys—termed the Rattlers and the Eagles—took part in a week-long tournament. During this time, their negativity culminated in derogatory name-calling, fistfights, and even vandalism and destruction of property. However, this work also revealed that such tension...
14.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety considerations for cereblon-recruiting targeted protein degraders.

Nature reviews. Drug discovery·2026
Same author

Ligandability assessment of interferonopathy-associated proteins using chemoproteomics.

British journal of pharmacology·2025
Same author

Expanding the druggable zinc-finger proteome defines properties of drug-induced degradation.

Molecular cell·2025
Same author

Property-Based Optimization of Cereblon-Based Molecular Glue Degraders.

ACS medicinal chemistry letters·2025
Same author

Introduction to the themed collection on 'Induced-Proximity Pharmacology'.

RSC medicinal chemistry·2025
Same author

Advances in sulfonyl exchange chemical biology: expanding druggable target space.

Chemical science·2025
Same journal

Targeting developmental reprogramming: hPSC insights for cancer interception.

Trends in pharmacological sciences·2026
Same journal

July 2026 issue first authors.

Trends in pharmacological sciences·2026
Same journal

Chronobiomaterials for circadian-aligned brain therapeutics.

Trends in pharmacological sciences·2026
Same journal

Biosensors for translatable GPCR bias.

Trends in pharmacological sciences·2026
Same journal

ECM stiffness and epigenetics in organ fibrosis.

Trends in pharmacological sciences·2026
Same journal

Which HTT transcript to lower?

Trends in pharmacological sciences·2026
See all related articles

Related Experiment Video

Updated: Jul 16, 2025

New Variations for Strategy Set-shifting in the Rat
09:45

New Variations for Strategy Set-shifting in the Rat

Published on: January 23, 2017

8.2K

ReSPONDINg TACtically, degrading strategically.

Breanna L Zerfas1, Lyn H Jones1

  • 1Center for Protein Degradation, Dana-Farber Cancer Institute, Boston, MA, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA, USA.

Trends in Pharmacological Sciences
|September 9, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed R-spondin chimeras (ROTACs) to degrade membrane proteins like PD-L1. This targeted protein degradation strategy offers a new platform for developing therapeutics against challenging membrane protein targets.

Keywords:
membrane proteinstargeted protein degradation

More Related Videos

Integrated Compensatory Responses in a Human Model of Hemorrhage
07:57

Integrated Compensatory Responses in a Human Model of Hemorrhage

Published on: November 20, 2016

12.6K
A Modified Lean and Release Technique to Emphasize Response Inhibition and Action Selection in Reactive Balance
07:19

A Modified Lean and Release Technique to Emphasize Response Inhibition and Action Selection in Reactive Balance

Published on: March 19, 2020

5.9K

Related Experiment Videos

Last Updated: Jul 16, 2025

New Variations for Strategy Set-shifting in the Rat
09:45

New Variations for Strategy Set-shifting in the Rat

Published on: January 23, 2017

8.2K
Integrated Compensatory Responses in a Human Model of Hemorrhage
07:57

Integrated Compensatory Responses in a Human Model of Hemorrhage

Published on: November 20, 2016

12.6K
A Modified Lean and Release Technique to Emphasize Response Inhibition and Action Selection in Reactive Balance
07:19

A Modified Lean and Release Technique to Emphasize Response Inhibition and Action Selection in Reactive Balance

Published on: March 19, 2020

5.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Targeted protein degradation is a key strategy for expanding the druggable proteome.
  • Therapeutic development for membrane proteins remains challenging due to their complex structures and limited accessibility.
  • Existing methods for targeting membrane proteins are insufficient for broad therapeutic application.

Purpose of the Study:

  • To develop a novel therapeutic platform for targeted degradation of membrane proteins.
  • To investigate the efficacy of R-spondin chimeras (ROTACs) in mediating protein degradation.
  • To establish a modular system for targeting various membrane proteins, including PD-L1.

Main Methods:

  • Development of R-spondin chimeras (ROTACs) by fusing R-spondin fragments with PD-L1 targeting moieties.
  • Utilizing the ubiquitin-proteasome system and lysosomal pathways for protein degradation.
  • Assessing the degradation efficiency of PD-L1 using biochemical and cellular assays.

Main Results:

  • ROTACs effectively induced lysosomal degradation of PD-L1.
  • The ROTAC platform demonstrated modularity and applicability to other membrane proteins.
  • Successful degradation of PD-L1 was confirmed through various experimental validations.

Conclusions:

  • ROTACs represent a promising new strategy for targeted degradation of membrane proteins.
  • This platform offers a versatile approach to overcome limitations in developing therapeutics for membrane protein targets.
  • The findings open avenues for broader applications of targeted protein degradation in medicine.