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Engineering pedicled vascularized bladder tissue for functional bladder defect repair
Mingming Yu1,2,3, Jiasheng Chen1,2,3, Lin Wang1,2,3
1Department of Urology Shanghai Children's Hospital, Shanghai Jiao Tong University Shanghai China.
Bioengineering & Translational Medicine
|September 11, 2023
Summary
This study demonstrates that integrating buccal mucosa grafts with vascularized smooth muscle tissue creates a functional, full-layer bladder construct. This engineered bladder shows promise for effective bladder reconstruction and functional recovery.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Urology
Background:
- Engineered bladder constructs aim to replicate native bladder structure and function for substitution therapies.
- Previous work established pedicled vascularized smooth muscle tissue for bladder reconstruction.
Purpose of the Study:
- To assess the integration of buccal mucosa graft (BMG) with vascularized smooth muscle tissue.
- To develop a full-layer, pedicled, vascularized bladder construct for bladder reconstruction.
Main Methods:
- Fabrication of a pedicled vascularized bladder construct using BMG and vascularized smooth muscle tissue.
- Reconstruction of full-thickness bladder defects using different constructs (pedicled capsule, nonpedicled, pedicled).
- Histological (Picrosirius Red staining, immunohistochemistry) and functional assessments (compliance, ductility).
Main Results:
- BMG integrated successfully, forming a full-layer construct with good survival, vascularization, and barrier function.
- The pedicled construct group exhibited minimal fibrosis, high smooth muscle proportion, and dense vascularization.
- Only the pedicled construct group showed a continuous mucosal layer, improved bladder compliance, and ductility.
Conclusions:
- Buccal mucosa graft integration with vascularized smooth muscle tissue yields a promising pedicled, vascularized bladder construct.
- This approach facilitates structural remodeling and functional recovery, offering an alternative for bladder reconstruction.
